The expression of Transient receptor potential melastatintype 2(TRPM2) in the immunocytes of sepsis patients and the role of TRPM2 in the development of sepsis
Not Applicable
Recruiting
- Conditions
- sepsisInflammatory and Immune System - Other inflammatory or immune system disorders
- Registration Number
- ACTRN12612001114831
- Lead Sponsor
- Xiangming Fang
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- All
- Target Recruitment
- 90
Inclusion Criteria
sepsis patients including sepsis, severe sepsis and sepsis shock patients.
Exclusion Criteria
Not sepsis patients
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method To detect the expression of TRPM2 Messenger ribonucleic acid (mRNA) in the monocyte by using real-time Polymerase Chain Reaction (PCR).[Within 24 hours after the diagnosis of sepsis in Intensive Care Unit (ICU). We only draw blood from the sepsis patients once.];To detect the expression of TRPM2 mRNA in the lymphocyte by using real-time PCR.[Within 24 hours after the diagnosis of sepsis in Intensive Care Unit (ICU). We only draw blood from the sepsis patients once.]
- Secondary Outcome Measures
Name Time Method infammatory cytokines (TNFa, IL-6, IL-8, IL-10) in the plasma of sepsis patients[Within 24 hours after the diagnosis of sepsis in ICU. We only draw blood from the sepsis patients once.];To detect immunocyte apoptosis in sepsis patients by using flow cytometer.[Within 24 hours after the diagnosis of sepsis in ICU. We only draw blood from the sepsis patients once.];To assess the physiology and health of patients by using acute physiology and chronic health evaluation (APACHE )score.<br><br>Using sepsis-related Organ Failure (SOFA) score, the organ damage of sepsis patients will be assessed.[Within 24 hours after the diagnosis of sepsis in ICU. We assess the scores only once.]