Mitochondrial Health and Muscle Function in Elderly
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 59
- 试验地点
- 1
- 主要终点
- Rate of ex vivo mitochondrial State 3 respiration
研究概览
简要总结
The aim of this study is to characterize the relation between skeletal muscle mitochondrial metabolism and muscle health in elderly, physically compromised humans. To study this relation, a cross-sectional study will be performed in well-defined, distinct subject groups. Thus, to obtain insight in the relation between mitochondrial health and muscle function, not only subjects that differ in mitochondrial function (based on physical activity) will be compared but also subjects with high- versus low muscle function will be selected.
详细描述
Aging is associated with a decline of mitochondrial and skeletal muscle volume, -quality and -function. If a causal link exists between the loss of mitochondrial function and muscle health is unknown, however, both appear with advancing age and are associated with the loss of functional capacity, which increases comorbidities and annual healthcare costs. The aim is to test the hypothesis that a compromised muscle function in sedentary elderly is related to an impaired mitochondrial health. A detailed characterization of mitochondrial metabolism and muscle function is performed in well-defined, (physically compromised) elderly humans, in a cross-sectional design. To obtain insight in the relation between mitochondrial health and muscle function, not only elderly subjects that differ in mitochondrial function (based on physical activity) will be compared but also elderly subjects with high versus low muscle physical function will be selected. Healthy, young (20-30 years,) individuals with normal physical activity levels will be included as absolute controls.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 65 Years 至 80 Years（Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males and females
- •Subject should be in sufficient health to participate in the experimentations, to be judged by the responsible MD based on the subject's medical history
- •Caucasian origin (see study groups)
排除标准
- •Any contraindications for MRI scan:
- •Aneurysm clips Implanted neural stimulator Implanted cardiac pacemaker of defibrillator Cochlear implant Iron- containing corpora aliena in the eye or brain Artificial (heart) valves which is contraindicated for MRS Claustrophobia
- •Diagnosed with diabetes mellitus
- •Poor health as judged by the responsible medical doctor
- •Heart problems: In case of an abnormal ECG in rest, this will be discussed with both the participant and the responsible medical doctor
结局指标
主要结局
Rate of ex vivo mitochondrial State 3 respiration
时间窗: Day 4
Ex vivo mitochondrial capacity, ADP-stimulated respiration expressed by O2flux in pmol/mg wet weight/second
次要结局
- Insuline sensitivity(Day 1)
- Body composition(Day 3)
- Muscle strength in upper-leg(Day 3)
- Muscle metabolism in vivo (H-MRS acetylcarnitine)(Day 5)
- Muscle volume upper-leg(Day 5)
- Physical functionality, 6MWT(Day 2)
- VO2max(Day 2)
- Dynamic gait stability(Day 3)
- Muscle oxidative capacity in vivo (PCr-MRS recovery)(Day 5)
- Energy expenditure in rest and during sub-maximal exercise(Day 4)