Study to Identify Biomarkers for Protein Intake
- Conditions
- Identify New Biomarkers in Blood or Urine After Consumption of Meat Protein, Dairy Protein and Grain Protein in Healthy Subjects.
- Interventions
- Other: Run in dietOther: High meat protein dietOther: High dairy protein dietOther: High grain protein diet
- Registration Number
- NCT01314040
- Lead Sponsor
- Wageningen University
- Brief Summary
Results from observational studies suggest a small beneficial association between protein and blood pressure that may be mainly attributable to the intake of protein from plant sources. In epidemiological studies biomarkers of dietary intake are useful to estimate intake more reliably. Dietary studies using biomarkers of protein intake generally show stronger associations with health outcomes. However, data on biomarkers for specific types of protein (e.g. from dairy, meat, grain and legumes) are scarce. Therefore the purpose of this study is to identify new biomarkers for the intake of protein from meat, dairy and grain. In addition the secondary purpose is to investigate whether intake of protein from these sources influences kidney filtration rate and urinary acid excretion differentially.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 25
- Age 18-40 years
- BMI between 18.5 and 30 kg/m2
- Healthy (As judged by the participant)
- Written informed consent obtained
- Unable or unwilling to comply with study procedures.
- Unusual dietary patterns, including high alcohol intakes (≥ 2 glasses/day) and a vegetarian diet
- Recent (< 4 weeks) or current participation in a study with any investigational drug or dietary intervention
- Usage of an energy restricted diet during the last two months
- Weight loss or weight gain of 5 kg or more during the last two months
- Smoking
- Stomach or bowel diseases
- Kidney disorders
- Diabetes, thyroid disease, other endocrine disorders
- Prevalent cardiovascular disease
- Extreme sports (>8h/ wk)
- Usage of any prescribed medication except for oral contraceptives
- Use of systemic antibiotics in the period of 3 month prior to the study
- For women: pregnant or lactating
- For women: not using oral contraceptives
- For women: Unwilling to use oral contraceptives for consecutively for at least the total study duration
- Liver function parameters (ALAT, ASAT, and γ-GT) and renal function parameters (Serum creatinin) outside the normal range
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Run in Run in diet - High meat protein diet High meat protein diet - High dairy protein diet High dairy protein diet - High grain protein diet High grain protein diet -
- Primary Outcome Measures
Name Time Method Differences between dietary periods in concentrations of potential biomerkers in blood and urine On the last day of every one week intervention period Potential biomarkers that will be measured are urinary creatinin, sulphate, carnosine, 1-MH, 3-MH, taurine,14N/15N and creatin in blood. Furthermore, amino acid profiling will be done in blood and urine to detect differences between dietary periods
- Secondary Outcome Measures
Name Time Method Differences between dietary period in estimated renal filtration blood and urine collection on the last day of each intervention period Renal filtration will be estimated using bromide distribution in combination with bio-impedance measurement
Differences between dietary periods in urinary acid excretion urine collection on the last day of each intervention period
Trial Locations
- Locations (1)
Wageningen University
🇳🇱Wageningen, Gelderland, Netherlands