A Phase III, Safety, Tolerability and Efficacy of Combination Treatment of BL-8040 and Granulocyte Colony Stimulating Factor (G-CSF) as Compared to Placebo and G-CSF for the Mobilization of Hematopoietic Stem Cells for Autologous Transplantation in Subjects With Multiple Myeloma (MM)

Phase 3

Active, not recruiting

Conditions: Multiple Myeloma

Interventions: Drug: BL-8040 1.25 mg/kg + G-CSF
Drug: Placebo +G-CSF

Registration Number: NCT03246529

Lead Sponsor: BioLineRx, Ltd.

Brief Summary: A total of 122 subjects were randomized into the study and investigated in the double-blind placebo-controlled setting to assess the efficacy and safety of G-CSF + BL-8040 as compared to G-CSF + placebo.

Detailed Description: * Part 1: This lead-in period, designed to ascertain the dose of BL-8040, enrolled a total of 12 subjects to an open labeled treatment to assess the efficacy, safety, pharmacokinetic (PK) and pharmacodynamic (PD) parameters of treatment with G-CSF 10 µg/kg/day and BL-8040 1.25 mg/kg, per study protocol to goal collection of ≥ 6 × 10\^6 CD34+ cells/kg.

* Part 2: Following the successful completion of Part 1, a total of 122 subjects were randomized into Part 2 of the study which employed a double-blind placebo-controlled setting to assess the efficacy and safety of G-CSF + BL-8040 as compared to G-CSF + placebo.

Recruitment & Eligibility

Status: ACTIVE_NOT_RECRUITING

Sex: All

Target Recruitment: 180

Inclusion Criteria

Histologically confirmed Multiple Myeloma prior to enrolment and randomization.
At least 1 week (7 days) from last induction cycle of combination/multi-agent cyto-reductive chemotherapy (e.g., KRD [carfilzomib, lenalidomide, dexamethasone] or VRD (e.g., bortezomib, lenalidomide, dexamethasone) or last single agent chemotherapy (e.g., lenalidomide, pomalidomide, bortezomib, dexamethasone, etc.) prior to the first dose of G-CSF for mobilization.
Eligible for autologous hematopoietic stem cell transplantation according to the Investigator's discretion.
The subjects should be in first or second CR (including CR and SCR) or PR (including PR and VGPR).
Eastern Cooperative Oncology Group (ECOG) performance status 0 or 1.
Adequate organ function at screening as defined as below:
1. Hematology:
  - White blood cell counts more than 2.5 x 10^9/L
  - Absolute neutrophil count more than 1.5 x 10^9/L
2. Platelet count more than 100 x10^9/L Renal Function:
  
  • Glomerular Filtration Rate (GFR) value of ≥15 mL/min/1.732 calculated by Modification of Diet in Renal Disease (MDRD) equation
3. Hepatic function:
  - Alanine aminotransferase (ALT) and/or aspartate aminotransferase (AST) ≤ 2.5 x ULN
  - Total Bilirubin ≤ 2.0 x Upper Limit Normal (ULN) unless the subject has Gilbert disease
4. Coagulation test:
  - International Normalized Ratio (INR) or Prothrombin Time (PT): ≤1.5 x ULN unless subject is receiving anticoagulant therapy, as long as PT or Partial Thromboplastin Time (PTT) is within therapeutic range of intended use of anticoagulants
  - Activated Partial Thromboplastin Time (aPTT): ≤1.5 x ULN unless subject is receiving anticoagulant therapy, as long as PT or PTT is within therapeutic range of intended use of anticoagulants
Male subjects must agree to use an adequate method of contraception starting with the first day of G-CSF administration through 30 days after the last dose of study drug.
Patients must have a signed study informed consent prior to entering the study.

Exclusion Criteria

Previous history of autologous or allogeneic-Hematopoietic Cell Transplantation (HCT).
Failed previous Hematopoietic Stem Cell (HSC) collections or collection attempts.
Taken any of the listed below concomitant medications, growth factors or stimulating agents within the designated washout period:
1. Dexamethasone: 7 days;
2. Thalidomide: 7 days;
3. Lenalidomide: 7 days;
4. Pomalidomide: 7 days;
5. Bortezomib: 7 days;
6. Carfilzomib: 7 days;
7. G-CSF: 14 days;
8. Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) or Neulasta®: 21 days;
9. Erythropoietin or erythrocyte stimulating agents: 30 days;
10. Eltrombopag, romiplostim or platelet stimulating agents: 30 days;
11. Carmustine (BCNU): 42 days/6 weeks;
12. Daratumumab: 28 days;
13. Ixazomib: 7 days.
Received >6 cycles lifetime exposure to thalidomide or lenalidomide.
Received >8 cycles of alkylating agent combinations.
Received >6 cycles of melphalan.
Received prior treatment with radioimmunotherapy (e.g., radionuclides, holmium).
Received prior treatment with venetoclax.
Plans to receive maintenance treatment within 60 days post-engraftment (e.g., lenalidomide, bortezomib, pomalidomide, thalidomide, carfilzomib, etc.)
Has received a live vaccine within 30 days of the planned start of G-CSF administration. Seasonal flu vaccines that do not contain live virus are permitted.
Known active central nervous system (CNS) metastases or carcinomatous meningitis.
A history of allergic reactions attributed to compounds of similar chemical or biologic composition to BL-8040, G-CSF, or other agents used in the study.
Has an active infection requiring systemic therapy or uncontrolled infection.
Has a known additional malignancy that is progressing or requires active treatment.
Has an underlying medical condition that would preclude study participation.
Is currently participating and receiving study therapy or has participated in a study of an investigational agent and received study therapy or used an investigational device within 4 weeks of the first dose of treatment.
O2 saturation < 92% (on room air).
Personal history or family history of Long QT Syndrome or Torsade de Pointes.
History of unexplained syncope, syncope from an uncorrected cardiac etiology, or family history of sudden cardiac death.
Myocardial infarction, coronary artery bypass grafting (CABG), coronary or cerebral artery stenting and /or angioplasty, stroke, cardiac surgery, or hospitalization for congestive heart failure within 3 months or greater than Angina Pectoris Class >2 or New York Heart Association (NYHA) Heart Failure >2.
ECG in screening showing QTcF > 470 msec, and/or PR > 280 msec,.
Mobitz II 2nd degree Atrioventricular (AV) Block, 2:1 AV Block, High Grade AV Block, or Complete Heart Block, unless the patient has an implanted pacemaker or implantable cardiac defibrillator (ICD) with backup pacing capabilities.
Has a history or current evidence of any condition, therapy, or laboratory abnormality that might confound the results of the trial, interfere with the subject's participation for the full duration of the trial, or is not in the best interest of the subject to participate, in the opinion of the treating investigator.
Has known psychiatric or substance abuse disorders that would interfere with cooperation with the requirements of the trial.
Is pregnant or breastfeeding, or expecting to conceive within the projected duration of the trial, starting with the screening visit through 30 days after the last dose of study drug.
Has a known history of HIV (HIV 1/2 antibodies)
Has known active Hepatitis B (e.g., Hepatitis B Surface Antigen [HBsAg] reactive) or Hepatitis C (e.g., Hepatitis C Virus [HCV] RNA [qualitative] is detected).
Untreated or unsuccessfully treated Hepatitis B or C.

Study & Design

Study Type: INTERVENTIONAL

Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
BL-8040 1.25 mg/kg + G-CSF	BL-8040 1.25 mg/kg + G-CSF	Double-blind placebo-controlled setting designed to assess the safety, tolerability and efficacy of G-CSF + BL-8040 as compared to G-CSF + Placebo, for stem cell mobilization in MM.
Placebo + G-CSF	Placebo +G-CSF	Double-blind placebo-controlled setting designed to assess the safety, tolerability and efficacy of G-CSF + BL-8040 as compared to G-CSF + Placebo, for stem cell mobilization in MM.

Primary Outcome Measures

Name	Time	Method
Percentage of Subjects Mobilizing ≥6 × 10^6 CD34+ Cells/kg With up to 2 Apheresis Sessions	From first day of study treatment (G-CSF) until day of second apheresis which was planned to occur on Day 6	Percentage of subjects mobilizing ≥6 × 10\^6 CD34+ cells/kg with up to 2 apheresis sessions in preparation for autologous hematopoetic cell transplantation (auto-HCT) after treatment with G-CSF + single administration of BL-8040/placebo. Based on central laboratory data.

Secondary Outcome Measures

Name	Time	Method
Percentage of Subjects Mobilizing ≥2 × 10^6 CD34+ Cells/kg in 1 Apheresis Session	From first day of study treatment (G-CSF) until day of first apheresis which was planned to occur on Day 5	Percentage of subjects mobilizing ≥2 × 10\^6 CD34+ cells/kg in 1 apheresis session after treatment with G-CSF + single administration of BL-8040/ placebo.
Time to Neutrophil Engraftment, After Auto-HCT	End of engraftment period, which was defined as 29 days post transplantation	Time to neutrophil engraftment after auto-HCT, where engraftment was defined as absolute neutrophil count (ANC) ≥0.5 × 10\^9/L for 3 days or ≥1.0 × 10\^9/L for 1 day following the conditioning regimen associated nadir.
Time to Platelet Engraftment, After Auto-HCT	End of engraftment period, which was defined as 29 days post transplantation	Time to platelet engraftment, after auto-HCT, where engraftment was defined as the first of 3 consecutive measurements of platelet count ≥20 × 10\^9/L without platelet transfusion support for 7 days following the conditioning regimen associated nadir.
Graft Durability at 6 Months Post Transplantation	6 Months Post Transplantation	Comparability of the graft durability between the BL-8040 + G-CSF arm and the placebo + G-CSF arm at 6 months post transplantation
Subjects With Graft Durability at 100 Days Post Transplant/ Early Termination	Day 100 Post-Transplantation (± 7 days)	Subjects achieving graft durability were defined as meeting the following 2 criteria: * Platelet count ≥50 × 10\^9/L without transfusion for at least 2 weeks. * Hemoglobin level ≥10 g/dL with no erythropoietin support or transfusions for at least 1 month. This analysis was performed in part 2 of the study only.
Graft Durability at 9 Months Post Transplantation	9 Months Post Transplantation	Comparability of the graft durability between the BL-8040 + G-CSF arm and the placebo + G-CSF arm at 9 months post transplantation
Graft Durability at 12 Months Post Transplantation	12 Month Post Transplantation	Comparability of the graft durability between the BL-8040 + G-CSF arm and the placebo + G-CSF arm at 12 months post transplantation
Percentage of Subjects Mobilizing ≥6 × 10^6 CD34+ Cells/kg in 1 Apheresis Session	From first day of study treatment (G-CSF) until day of first apheresis which was planned to occur on Day 5	Percentage of subjects mobilizing ≥6 × 10\^6 CD34+ cells/kg in 1 apheresis session after treatment with G-CSF + single administration of BL-8040/ placebo.

Trial Locations

Locations (18): UCLA Medical Center
🇺🇸
Los Angeles, California, United States
Loyola University Medical Center
🇺🇸
Chicago, Illinois, United States
University of Debrecen
🇭🇺
Debrecen, Hungary
MD Anderson Cancer Center
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Houston, Texas, United States
University of Miami
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Miami, Florida, United States
University of Maryland
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Baltimore, Maryland, United States
Hospital Universitario 12 de Octubre
🇪🇸
Madrid, Spain
Presidio Ospedaliero Morelli Viale Europa
🇮🇹
Reggio Calabria, Italy
Hospital de La Santa Creu I Sant Pau
🇪🇸
Barcelona, Spain
Hospital University Ramon y Cajal
🇪🇸
Madrid, Spain
The Washington University School of Medicine
🇺🇸
Saint Louis, Missouri, United States
Div. Clinicizzata di Ematologia - Policlinico Vittorio Emanuele
🇮🇹
Catania, Italy
University of Koln
🇩🇪
Köln, Koln, Germany
University of Cincinnati
🇺🇸
Cincinnati, Ohio, United States
Huntsman Cancer Institute in University of Utah
🇺🇸
Salt Lake City, Utah, United States
Mayo Clinic
🇺🇸
Rochester, Minnesota, United States
Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases
🇭🇺
Budapest, Hungary
University of Florida
🇺🇸
Gainesville, Florida, United States