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Dose dependent acute effects of decaffeinated coffee on endothelial function and glucose metabolism in healthy volunteers

Completed
Conditions
Cardiovascular and metabolic diseases/ clinical nutrition
Nutritional, Metabolic, Endocrine
N/A
Registration Number
ISRCTN41583899
Lead Sponsor
niversity of Palermo (Italy)
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
All
Target Recruitment
15
Inclusion Criteria

1. Male and female healthy subjects
2. Range of age: 25-50 years
3. Range of body mass index (BMI): 20-28 kg/m^2

Exclusion Criteria

1. Any metabolic, cardiovascular or systemic disease
2. Any drug treatment
3. Smoking habits
4. Pregnancy or lactation in the last six months

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
1. Fasting acute effects of the two different doses of coffee on endothelial function, measured as the flow mediated dilation (FMD). FMD measurements (outcome assessors blinded) will be performed before, 30 and 60 minutes after coffee ingestion.<br>2. Fasting acute effects of the two different doses of coffee on glucose metabolism, evaluated on the basis of blood concentrations of glucose, insulin and c-peptide. These blood measurements will be obtained before and 60 minutes after coffee ingestion.
Secondary Outcome Measures
NameTimeMethod
1. Blood pressure, obtained before, 30 and 60 minutes after coffee ingestion <br>2. Heart rate, measured continuously for the entire duration of the test (60 min) <br>3. Electrocardiogram with QTc interval measurement, obtained before and 60 minutes after coffee ingestion <br>4. A blood sample will be collected before and 60 minutes after coffee ingestion in both occasions and the serum samples will be frozen at -80°C for future measurements (Interleukin-6 [IL-6], tumour necrosis factor-alpha [TNF-alpha], etc.)
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