Effect of Herbs and Spices on Metabolic Regulation and Appetite in Healthy Subjects

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Dietary Supplement: Spices 1; Dietary Supplement: Spices 2; Dietary Supplement: Spices 3; Dietary Supplement: Control drink; Dietary Supplement: Herbs 1; Dietary Supplement: Herbs 2

• Registration Number: NCT02035241
• Lead Sponsor: Lund University

Brief Summary

The purpose of the study is to investigate how herbs and spices affect acute/postprandial glucose and insulin responses, inflammatory markers, appetite control peptides, antioxidative capacity, as well as subjective appetite ratings (VAS-visual analogue scales) in healthy volunteers. We hypothesize that certain herbs and spices added to a standardized meal will improve postprandial glucose tolerance and other metabolic biomarkers in healthy volunteers, compared with a similar meal without the corresponding plant materials.

Detailed Description

The aims of the study is to investigate whether consumption of preload isovolumetric (220 ml) spice-based beverages contained total polyphenol concentration to 185 mg gallic-acid equivalents followed by white wheat bread challenge (contained 50 g available carbohydrate) might affect postprandial glucose metabolism, inflammatory markers, appetite-related gut hormones, plasma antioxidant capacity, as well as appetite sensations in healthy subjects.

Study Timeline

• Study First Submitted: 2014-01-10
• Study First Submitted QC: 2014-01-10
• Study First Posted: 2014-01-14
• Study Start: 2014-03
• Primary Completion: 2014-06
• Study Completion: 2014-06
• Status Verified: 2018-08
• Last Update Submitted: 2018-08-23
• Last Update Posted: 2018-08-27

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 21

Inclusion Criteria

• Healthy males and females
• Signed informed consent
• BMI 20 - 28 kg/m2 with weight change <3 kg latest 2 months)
• Must be able to accept herbs and spices

Exclusion Criteria

• Below 18 years
• Uncomfortable speaking English and/or difficulties in understanding spoken English
• Smoking or using snuff
• Vegetarian or vegan
• Stressed by venous blood sampling or previous experience of being difficult to cannulate
• Receiving any drug treatment that may influence the study outcomes
• Pregnancy or breastfeeding

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Spices 1	Spices 1	220 ml test drink containing spices 1, acute study / one time administration
Spices 2	Spices 2	220 ml test drink containing spices 2, acute study / one time administration
Spices 3	Spices 3	220 ml test drink containing spices 3, acute study / one time administration
Placebo	Control drink	220 ml control drink, acute study / one time administration
Herbs 1	Herbs 1	220 ml test drink containing herbs 1, acute study / one time administration
Herbs 2	Herbs 2	220 ml test drink containing herbs 2, acute study / one time administration

Primary Outcome Measures

Name	Time	Method
Changing on blood glucose concentration after treatment with herbs and spices	Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min.	The capillary blood samples will be taken for blood glucose analysis

Secondary Outcome Measures

Name	Time	Method
Changing on subjective appetite ratings after treatment with herbs and spices	Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min.	Appetite will be assessed using standard subjective 100 mm VAS (visual analogue scale) at time intervals throughout each visit
Changing on metabolic biomarkers after treatment with herbs and spices	Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min.	The venous blood samples will be taken for insulin, inflammatory markers (i.e. interleukin-6, interleukin-8, tumor necrosis factor-alpha, necrosis factor kappa-beta, adiponectin and C-reactive protein), appetite-related gut hormones (i.e. glucagon-like peptide-1 and peptide tyrosine-tyrosine)
Changing on urine metabolite profile after treatment with herbs and spices	Prior to the initial intervention at 0 min and up to 24-h after intervention	Metabolite profile will be analyzed using Liquid Chromatography-Mass Spectrometry based methods
Changing on plasma metabolite profile after treatment with herbs and spices	Prior to the initial intervention at 0 min and after intervention at 60, 90, 120, 180 min.	Metabolite profile will be analyzed using Liquid Chromatography-Mass Spectrometry based methods

Trial Locations

Locations (1)

Antidiabetic Food Centre (Medicon Village) - Lund University; 🇸🇪 Lund, Skane, Sweden