Day-night Rhythm in Muscle Metabolism of Prediabetic Subjects
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Diabetes Mellitus, Type 2
- Sponsor
- Maastricht University
- Enrollment
- 12
- Locations
- 1
- Primary Endpoint
- Day-night rhythm in skeletal muscle mitochondrial respiration
- Status
- Completed
- Last Updated
- 6 years ago
Overview
Brief Summary
This study evaluates the existence of a day-night rhythm in skeletal muscle energy metabolism in prediabetic subjects. Subjects will stay at the research facility for 44 hours with a standardized living protocol during which several measurements of skeletal muscle and whole body energy metabolism will be performed.
Detailed Description
Recent evidence from both observational and experimental studies indicates that disobedience of a normal day-night rhythm negatively influences metabolic and cardiovascular health, possibly leading to obesity and type 2 diabetes mellitus (T2DM). An important hallmark of obesity and T2DM is a decreased skeletal muscle mitochondrial function. Interestingly, recent research indicates that also skeletal muscle mitochondrial function is under the influence of a day-night rhythm. In this study, the presence of a day-night rhythm in skeletal muscle energy metabolism (in particular mitochondrial function) of overweight prediabetic participants will be investigated. During this study, subjects will stay at the research unit for 44 hours, with standardized meals and sleeping time. Five muscle biopsies (v. lateralis) and frequent blood draws will be taken. In addition, indirect calorimetry measurements will be performed.
Investigators
Eligibility Criteria
Inclusion Criteria
- Not provided
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Day-night rhythm in skeletal muscle mitochondrial respiration
Time Frame: measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00
O2-flux (pmol/mg/s) measured with high resolution respirometry
Secondary Outcomes
- Day-night rhythm in whole-body energy metabolism (energy expenditure)(measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00)
- Day-night rhythm in serum free fatty acids (FFA)(measured on day two, every two hours for 24 hours beginning at 08:00)
- Day-night rhythm in whole-body energy metabolism (glucose oxidation)(measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00)
- Day-night rhythm in muscle DNA involved in molecular clock and mitochondrial metabolism.(measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00)
- Day-night rhythm of in markers of normal day-night rhythm (melatonin)(measured on day two, every two hours for 24 hours beginning at 08:00)
- Day-night rhythm in serum triglycerides(measured on day two, every two hours for 24 hours beginning at 08:00)
- Day-night rhythm in serum cholesterol(measured on day two, every two hours for 24 hours beginning at 08:00)
- Change in energy status of the heart between morning and evening(Measured on first study day 5:30 PM (duration 45 minutes), second study day 7:30 AM (duration 45 minutes))
- Day-night rhythm in whole-body energy metabolism (fat oxidation)(measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00)
- Day-night rhythm in muscle protein levels of markers involved in molecular clock and mitochondrial metabolism.(measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00)
- Day-night rhythm in serum glucose(measured on day two, every two hours for 24 hours beginning at 08:00)
- Day-night rhythm in muscle DNA of markers involved in molecular clock and mitochondrial metabolism.(measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00)
- Day-night rhythm in muscle mRNA markers involved in molecular clock and mitochondrial metabolism.(measured on day two at 8:00, 13:00, 18:00, 23:00, 04:00)
- Day-night rhythm of markers of normal day-night rhythm (cortisol)(measured on day two, every two hours for 24 hours beginning at 08:00)
- Day-night rhythm of in markers of normal day-night rhythm (core body temperature)(measured continuously for 24 hours on day two)
- Day-night rhythm in serum insulin(measured on day two, every two hours for 24 hours beginning at 08:00)