MedPath

Quantitative Muscle Ultrasound as a Marker of Progression in Children With Muscular Diseases

Completed
Conditions
Duchenne Muscular Dystrophy
Inflammatory Myopathy
Interventions
Diagnostic Test: Quantitative muscle ultrasound measurements
Registration Number
NCT03786913
Lead Sponsor
Benha University
Brief Summary

The aim of our study is to Assess skeletal muscle structural status in children with inflammatory myositis and Duchenne muscular dystrophy using musculoskeletal ultrasound and to perform a longitudinal follow up of these changes over 2 years and to assess the relation between these findings with clinical parameters, functional scales, biochemical and electromyographic tests.

Detailed Description

This study will be carried out on two groups:

• Group (I): fifty children diagnosed to have duchenne muscular dystrophy and inflammatory myositis.

Group (II): including 20 healthy children matching age and sex as control group.

patients will be subjected to

(A) Clinical evaluation

1. Complete history taking.

2. Thorough clinical examination.

3. Body mass index (BMI) assessment.

4. Quantitative muscle strength tests

5. Functional grading

6. Childhood Myositis Assessment Scale. 7 (B) Laboratory assessment:

All patients will be subjected to the following measurements:

1. Serum creatine kinase levels (CK).

2. Serum Lactate dehydrogenase levels

3. Serum of Liver enzymes (SGOT\& SGPT) levels.

(C) Electromyographic (EMG) assessment:

(D) Musculoskeletal ultrasound assessment (E) Statistical analysis

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
48
Inclusion Criteria
  • children with Duchenne muscular dystrophy (DMD). Diagnosis with DMD was established according to DMD diagnostic criteria (Jennekens et al., 1991).
  • children with juvenile dermatomyositis (JDM) according to Bohan and Peter diagnostic criteria ( (Bohan and Peter, 1975).
Exclusion Criteria
  • Patients with age less than 2 years were excluded from the study due to inability to perform manual muscle testing and functional scales.
  • If no final diagnosis could be established.
  • The presence of a concomitant illness that may result in peripheral neuropathy or myopathy.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
children with muscle diseaseQuantitative muscle ultrasound measurementsfifty children diagnosed to have inflammatory myositis or Duchenne muscular dystrophy in whom Quantitative muscle ultrasound measurements will be performed .The captured images will be analyzed for echo intensity by means of computer-assisted grayscale histogram analysis at baseline and after 24 months.
control groupQuantitative muscle ultrasound measurements20 healthy children matching age and sex as control group in whom Quantitative muscle ultrasound measurement will be performed at baseline
Primary Outcome Measures
NameTimeMethod
Serum creatine kinase (CK) levels24 months

CK measured in U/L using ELISA

Serum Lactate dehydrogenase (LDH) levels24 months

CK measured in IU/L using ELISA

Aspartate aminotransferase (AST)24 MONTHS

AST measured in U/L using ELISA

alanine aminotransferase (ALT)24 months

ALT measured in U/L using ELISA

Kendall's manual muscle testing24 months

Kendall's 0 -10 point scale measures strength of each muscle group score 0 is the weakest (worst) and 10 is the strongest (best). The following muscles were tested bilaterally: the biceps brachii muscle (BB), the forearm flexors (FF), the rectus femoris muscle (RF), the tibialis anterior muscle (TA)

Childhood myositis assessment scale24 months

used to assess the severity of muscle involvement in children with dermatomyositis. The scores for the 14 items are summated to give a total score ranging from 0 (worst) to 52 (best)

motor unit potential (MUP) duration24 months

quantitative electromyography (QEMG) in the most affected rectus femoris and biceps brachii muscles will be performed and The motor unit potentials will be reviewed offline for the needle-detected EMG signals will be analyzed by the device software for the MUP duration measured in milliseconds.

motor unit peak-to-peak amplitude24 months

quantitative electromyography (QEMG) in the most affected rectus femoris and biceps brachii muscles will be performed and The motor unit potentials will be reviewed offline for the needle-detected EMG signals will be analyzed by the device software for the peak-to-peak amplitude measured in microvolt

motor unit area to amplitude ratio (AAR)24 months

quantitative electromyography (QEMG) in the most affected rectus femoris and biceps brachii muscles will be performed and The motor unit potentials will be reviewed offline for the needle-detected EMG signals will be analyzed by the device software for the motor unit AAR .

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Benha University Hospital

🇪🇬

Banhā, Qalubiya, Egypt

© Copyright 2025. All Rights Reserved by MedPath