Digestion and Absorption Kinetics of Beef Consumed Alone or as Part of a Mixed-Meal: a Crossover Trial in Young Adults
Overview
- Phase
- Not Applicable
- Status
- Not yet recruiting
- Enrollment
- 10
- Locations
- 1
- Primary Endpoint
- Digestion and absorption kinetics
Overview
Brief Summary
The purpose of this study is to assess the digestion and absorption kinetics of beef consumed alone or as part in healthy adults. In a randomized, crossover design, 10 young, healthy adult males and females aged 18-35 years, will consume a meal consisting of 25 g protein from minced beef alone or as part of a mixed-meal during two separate experimental trials. The main study endpoint is the exogenous rate of amino acid appearance in response to the ingested beef over a 10-h period.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Aged between 18-35 years
- •BMI between 18.5-30 kg/m2
- •Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)
- •No physical limitations (i.e., able to perform all activities associated with daily living independently)
Exclusion Criteria
- •Vegan, vegetarian, or does not consume beef protein
- •Diagnosed musculoskeletal disorders
- •Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
- •Chronic use of gastric acid-suppressing medication or anti-coagulants
- •Unstable weight over the last three months
- •Diagnosed GI tract disorders or diseases
- •Blood donation in the past 2 months
- •Females: pregnancy
Arms & Interventions
Beef only
Intervention: Food proteins (Dietary Supplement)
Beef in mixed-meal
Intervention: Food proteins (Dietary Supplement)
Outcomes
Primary Outcomes
Digestion and absorption kinetics
Time Frame: Over the 10 hour timeframe following beef ingestion
The exogenous rate of amino acid appearance in response to the ingested beef (expressed as μmol/kg/min). Based on tracer plasma amino acid concentrations over time (measured with ultra performance liquid chromatography-mass spectrometry).
Secondary Outcomes
- Whole-body protein metabolism(Over the 10 hour timeframe following beef ingestion)