Post-exercise Dietary Protein Strategies

Not Applicable

Completed

• Conditions: Optimal Anabolic Nutrition Interventions
• Interventions: Other: whey protein bolus; Other: whey protein pulses

• Registration Number: NCT01319513
• Lead Sponsor: McMaster University

Brief Summary

Protein ingestion increases the rate at which the body builds new proteins in skeletal muscle (muscle protein synthesis. This study is designed to examine how the pattern of feeding affects muscle protein synthesis following resistance exercise. There is reason to believe that the large rapid increase in blood amino acid concentrations that accompanies the ingestion of a bolus of protein is important to increasing muscle protein synthesis. Thus, we hypothesize that the consumption a bolus of protein will elevate muscle protein synthesis to a greater extent than the consumption of an equivalent amount of protein that is consumed in small divided doses.

Detailed Description

The rapid appearance into the blood of essential amino acids, and leucine in particular, may act as an important signal to stimulate muscle protein synthesis after resistance exercise. This may explain why consuming rapidly-absorbed whey protein may have an anabolic edge over slowly-absorbed proteins such as casein. Previous investigations into importance of the rate of absorption to muscle protein synthesis that have used 'fast' and 'slow' proteins have been confounded by differences in amino acid composition. The present study addresses this issue by administering the same protein source, whey, as either a bolus or in small divided 'pulse' doses to achieve divergent amino acid profiles after a bout of resistance exercise.

This study is being conducted in young (18-35) men.

Our outcome measures include: blood amino acid concentrations, rates of myofibrillar protein synthesis, anabolic intracellular signalling markers

Study Timeline

• Study Start: 2009-07
• Primary Completion: 2009-10
• Study Completion: 2009-10
• Status Verified: 2011-03
• Study First Submitted: 2011-03-18
• Study First Submitted QC: 2011-03-18
• Last Update Submitted: 2011-03-18
• Study First Posted: 2011-03-21
• Last Update Posted: 2011-03-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 8

Inclusion Criteria

• Non-obese men (BMI <27) between the age of 18 and 35 yrs.

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Exclusion Criteria

• Type II diabetes or other known diseases
• Use of medication
• Female
• Other ages or BMI than indicated above
• Resistance training > 3X/wk

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
protein feeding	whey protein bolus	Participants will complete 2 trials in a cross-over fashion in which they will consume whey protein either as a single bolus or as 10 small divided doses
protein feeding	whey protein pulses	Participants will complete 2 trials in a cross-over fashion in which they will consume whey protein either as a single bolus or as 10 small divided doses

Primary Outcome Measures

Name	Time	Method
Rate of myofibrillar protein synthesis	4 months	Rates of myofibrillar protein synthesis will be measured from muscle biopsy samples obtained from subjects participating in the study protocol.

Secondary Outcome Measures

Name	Time	Method
Myocellular protein phosphorylation	4 months	Protein phosphorylation of target proteins will be measured from Western blot analysis of muscle biopsy samples.

Trial Locations

Locations (1)

Ivor Wynne Centre A103, McMaster University; 🇨🇦 Hamilton, Ontario, Canada