Protein Ingestion Timing on Body Composition and Biochemical Markers in Resistance-trained Males

Not Applicable

Not yet recruiting

• Conditions: Hypertrophy; Physical Performance
• Interventions: Dietary Supplement: isolate whey protein prior to and immediately after resistance training; Dietary Supplement: isolate whey protein 3 hours prior to and 3 hours after resistance training

• Registration Number: NCT05544955
• Lead Sponsor: Marymount University

Brief Summary

Protein ingestion timing is an important component for muscle accretion. We hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men.

Detailed Description

Not available

Study Timeline

• Status Verified: 2022-09
• Study First Submitted: 2022-09-12
• Study First Submitted QC: 2022-09-14
• Last Update Submitted: 2022-09-14
• Study Start: 2022-09-17
• Study First Posted: 2022-09-19
• Last Update Posted: 2022-09-19
• Primary Completion: 2022-12-31
• Study Completion: 2022-12-31

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: Male
• Target Recruitment: 40

Inclusion Criteria

• performing resistance training at least three times a week for 1 year prior to the start of the study
• not taking any steroids or any illegal agents known to increase muscle size for at least 1 year prior to the start of the study
• no known medical issues
• sleeping for at least seven to eight hours during the 24-hour day

Exclusion Criteria

• non-willingness to continue protein ingestion or exercise protocols
• participation in exercise other than the prescribed resistance training program
• consumption of dietary supplement (other than the protein intervention) during the study period
• Missing more than one resistance training session or protein ingestion throughout the study period

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Protein ingestion immediately prior to and immediately after resistance training	isolate whey protein prior to and immediately after resistance training	This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.
Protein ingestion 3 hours prior to and 3 hours after resistance training	isolate whey protein 3 hours prior to and 3 hours after resistance training	This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Primary Outcome Measures

Name	Time	Method
Body Composition	12 weeks	Total Lean mass, fat mass, and estimated VAT (g) will be assessed using whole-body DXA

Secondary Outcome Measures

Name	Time	Method
Muscular Strength	12 weeks	Using 1 repetition maximum
Pull-ups	12 weeks	Using the pull-up test
Muscular Endurance	12 weeks	Using leg- and chest press exercises at 75% of the 1-RM
Jump testing	12 weeks	Using the vertical and broad jump tests
Creatinine and Urea Nitrogen	12 weeks	Urine samples will be collected using standard procedures
Cholesterol Levels	12 weeks	Using Fasting blood samples
Liver enzymes (ALT, AST, and GGT)	12 weeks	Using Fasting blood samples

Trial Locations

Locations (1)

Marymount University; 🇺🇸 Arlington, Virginia, United States