Mycoprotein and Pea Protein Blend and Muscle Protein Synthetic Response

Not Applicable

Completed

• Conditions: Muscle Protein Synthesis
• Interventions: Dietary Supplement: Bolus ingestion of protein

• Registration Number: NCT04894747
• Lead Sponsor: University of Exeter

Brief Summary

Dietary protein is vital for the preservation of health and optimal adaptation to training. Plant proteins are considered inferior to animal proteins with respect to their ability to stimulate an acute muscle building response and therefore support long-term muscle reconditioning. Pea protein is a highly commercially available plant proteins source (available as supplements, food ingredients etc.), yet there is no research investigating its ability to stimulate a muscle building response. The investigators aim to assess the effect of consuming pea protein on muscle protein synthesis rates and compare these results to mycoprotein, a source known to elicit a robust anabolic response.

Pea protein is lower in some of the essential amino acids, namely methionine, which could mean it is less effective compared with mycoprotein which has a more complete amino acid profile. So in addition to comparing pea with mycoprotein, the investigators also want to compare to a blend of pea and mycoprotein to see if replenishing the amino acid content in pea 'rescues' the anabolic response.

Detailed Description

Not available

Study Timeline

• Study Start: 2021-05-01
• Study First Submitted: 2021-05-05
• Study First Submitted QC: 2021-05-19
• Study First Posted: 2021-05-20
• Primary Completion: 2022-06-15
• Study Completion: 2022-06-15
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-21
• Last Update Posted: 2023-03-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 33

Inclusion Criteria

• BMI between 18 and 30
• Resistance trained

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Exclusion Criteria

• Any metabolic impairment
• Smoking
• Use of over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices).
• A personal family history of epilepsy, seizures or schizophrenia.
• Allergic to Quorn/mycoprotein/edible fungi/environmental mould products.
• Any motor disorder.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Pea protein	Bolus ingestion of protein	Bolus ingestion of pea protein providing 25g of protein.
Mycoprotein	Bolus ingestion of protein	Bolus ingestion of mycoprotein providing 25g of protein.
Mycoprotein/pea protein dry blend	Bolus ingestion of protein	Bolus ingestion of mycoprotein/pea protein dry blend providing 25g of protein.

Primary Outcome Measures

Name	Time	Method
Muscle protein synthesis	8.5 hours	The rate of synthesis of new muscle protein (Fractional synthetic rate %/h)

Secondary Outcome Measures

Name	Time	Method
Serum insulin concentrations	8.5 hours	Basal and postprandial concentrations of serum insulin
mTOR phosphorylation via ATP kinase assay	8.5 hours	The amount of mTOR that has been activated in the muscle cell
Translocation of mTOR via immunohistochemistry	8.5 hours	Locality of mTOR within the muscle cell
Plasma amino acid kinetics	8.5 hours	The appearance of amino acids from the protein drink in the circulation.

Trial Locations

Locations (1)

University of Exeter; 🇬🇧 Exeter, United Kingdom