The Importance of Dietary Protein Quality for Skeletal Muscle Anabolism in Older Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 30
- 试验地点
- 2
- 主要终点
- Muscle architecture using ultrasound
研究概览
简要总结
Dietary proteins potently augment muscle protein synthesis. Because of poorer anabolic sensitivity with ageing, studies and guidelines recommend higher dietary protein intake for older adults. Although higher doses would benefit skeletal muscle remodelling, large protein consumption is not feasible for many older adults. To circumvent, high-protein quality which possesses a high amino acid profile and digestibility appears to have an emergent role for supporting anabolism. Since currently the best line of defence against age related muscle loss is resistance exercise training and regular protein consumption, emphasising high-quality protein ingestion, such as whey protein, within meals may be feasible and efficacious in supporting musculoskeletal remodelling in older adults, without requirement for large protein doses.
The investigators propose that at low doses, high quality protein will have additive benefit to muscle protein synthesis compared to low-quality protein. Further, combining high-quality protein diets with resistance exercise training will have more profound benefits for muscle protein synthesis and muscle remodelling more so than low-quality protein diets.
详细描述
Participants will be randomly assigned to a 10-day dietary intervention consuming primarily animal proteins (high-quality condition) or primarily plant proteins (low-quality condition). In both conditions, participants will undertake supervised single-leg resistance exercise training every other day, amassing five days resistance exercise sessions during the study. The groups will aim to be matched and counterbalanced for gender. Equally for unilateral exercise randomisation for leg dominance will aim to be counterbalanced within both groups.
Preliminary assessments:
5 days preceding the start of the dietary intervention, participants will report to the University of Birmingham, Sport, Exercise and Rehabilitation Science for the following:
- Health questionnaire
- Written informed consent
- Anthropometrics (Height and Weight)
- Body fat (bioelectrical impedance)
- Start measuring habitual activity (provided with an activity monitor and pedometer)
- Start measuring habitual diet (provided with diet diary)
2 days preceding the start of the dietary intervention, participants will report to the University of Birmingham, Sport, Exercise and Rehabilitation Science for the following:
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
盲法说明
The participant will not know which condition they are under due to the contribution of animal and plant based elements within the diet, the conditions are not immediately obvious. Further, the higher and lower quality supplemental drinks have been made so the taste and texture of which are not discernible.
The randomisation and counterbalancing will be done by a researcher separate to the project, using anonymised participant codes. The condition participants are under will be also completed before inducting participants. Researchers will be blinded to the condition and which leg was exercised during the analysis.
入排标准
- 年龄范围
- 50 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males and Females aged between 50-70 years
- •Accustomed to normal physical activity levels (averaging at least 7000 steps a day)
- •Healthy body mass (i.e., BMI 18-25 kg/m2)
- •General good health indicated by health questionnaire
- •Free from COVID-19 (SARS-CoV-2) symptoms.
排除标准
- •Habitually high consumers of protein (>1.6g/kg/day) assessed by habitual diet diary
- •Food allergies
- •Lidocaine allergy
- •Bleeding Disorders
- •Chronic/systemic illnesses (i.e., renal failure, rheumatoid arthritis, diabetes, poor lung function, heart disease, cancer, uncontrolled hypertension)
- •Regular consumption of any analgesic or anti-inflammatory drugs. Taking medications mknown to affect muscle metabolis (e.g. beta-blockers, corticosteroids).
结局指标
主要结局
Muscle architecture using ultrasound
时间窗: 0-10 days
Using non-invasive ultrasound to determine change of muscle structure with training between protein quality conditions.
Leg Strength using dynamometry
时间窗: 0-10 days
Evaluating change in leg strength, of trained leg, pre- and post-intervention with high-quality and low-quality protein condition
Neural activation using interpolated twitch
时间窗: 0-10 days
Assessing change in neural activation via non-invasive interpolated twitch technique of between exercised legs pre-and post intervention in both protein quality conditions
Muscle protein synthesis using muscle biopsies and deuterated water enrichment.
时间窗: 0-10 days
Muscle protein synthesis will be assessed during the high-quality and low-quality intervention and compared between exercised and controlled leg.
次要结局
- Appetite regulation using questionnaires(0-10 days (3 hours postprandial))
- Nitrogen balance from urine and dietary protein intake(0-10 days (24-hour urine collection))
- Physical activity levels using accelerometery and pedometer(-5-10 days)
- Intramuscular signalling using western blotting(0-10 days)
- Appetite regulation, blood(0-10 days (3 hours postprandial))
- Metabolic rate, metabolic cart(0-10 days)
- Body composition using Duel- energy x-ray absorptiometry (DXA) scan.(0-10 days)
- Muscle fibre specific analysis using immunofluorescence microscopy.(0-10 days)