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2015-Metabolomics&Microbiome-infertility

Recruiting
Conditions
Azoospermia, Nonobstructive
Interventions
Other: Analysis of microbiome and metabolomics
Registration Number
NCT06181851
Lead Sponsor
IRCCS San Raffaele
Brief Summary

The study aims to carry out a translational analysis of the microbiome and metabolomics in patients suffering from non-obstructive azoospermia, with the aim of investigating prognostic factors predictive of the possible finding of spermatozoa following testicular pulp extraction and differences in blood and seminal level with the fertile population to identify etiopathogenic pathways of this condition.

Detailed Description

The study aims to carry out a translational analysis of the microbiome and metabolomics in patients suffering from non-obstructive azoospermia, with the aim of investigating prognostic factors predictive of the possible finding of spermatozoa following testicular pulp extraction and differences in blood and seminal level with the fertile population to identify etiopathogenic pathways of this condition.

Furthermore, the study aims to assess if the blood and seminal alterations of infertile patients have similarities with the same fluids of elderly patients, validating from a metabolomic and microbiomic point of view the hypothesis of premature aging of infertile patients.

Recruitment & Eligibility

Status
RECRUITING
Sex
Male
Target Recruitment
45
Inclusion Criteria
  • diagnosis of idiopathic non-obstructive azoospermia
  • fertile men (pregnancy within 12 months from the start of the couple's search)
  • men known to have been fertile aged between 65 and 75 (pregnancy within 12 months of the couple starting searching)
Exclusion Criteria
  • diagnosis of varicocele
  • endocrine alterations
  • chromosomal alterations
  • known genetic alterations

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Infertile menAnalysis of microbiome and metabolomicsPatients diagnosed with idiopathic non-obstructive azoospermia
Fertile menAnalysis of microbiome and metabolomicsfertile subjects (pregnancy within 12 months of the start of the research by the couple, according to WHO 2010 criteria)
Old fertile menAnalysis of microbiome and metabolomicsSubjects known to have been fertile subjects (pregnancy within 12 months of the start of the research by the couple, according to WHO 2010 criteria)
Primary Outcome Measures
NameTimeMethod
Prognostic role of the blood and semen samplesBaseline

Metabolomics and microbiome signature fertile and infertile men predicting possible finding of spermatozoa

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

IRCCS Ospedale San Raffaele

🇮🇹

Milan, Lombardy, Italy

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