Comparison of quantity of DNA using various methods
- Conditions
- Health Condition 1: null- CHRONIC PERIODONTITIS
- Registration Number
- CTRI/2018/04/013284
- Lead Sponsor
- DR SHIVAPRASAD BM
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Open to Recruitment
- Sex
- Not specified
- Target Recruitment
- 0
1.All subjects willing to participate in the study.
2.Chronic periodontitis patients with 5 or more teeth with probing depth >=5mm and clinical attachment level >= 3mm and gingival index >=2 will be included .
3.All the patients should be systemically healthy and should not have received periodontal treatment for at least 6 months prior to the clinical examination and sampling.
Smokers.
Systemic diseases like diabetes mellitus, heart diseases ,rheumatoid arthritis, hepatitis and other systemic diseases that can alter the course of periodontal disease.
Subjects on any medication taken within the last 6 months which may alter the periodontal status.
Pregnant and lactating mothers.
Any periodontal therapy in the last 6 months
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Quantity of DNA obtained from Saliva and GCF,extracted using micropipettes,paper strips and paper pointsTimepoint: Quantity of DNA obtained from Saliva and GCF,extracted using micropipettes,paper strips and paper points were assessed immediately and after 1 and 3 months respectively
- Secondary Outcome Measures
Name Time Method Quantity of DNA obtained from Saliva and GCF, extracted using micropippetes, paper strips and paper pointsTimepoint: Quantity of DNA obtained from Saliva and GCF , extracted using micropippetes, paper strips and paper points were assessed immediately and after 1 and 3 months respectively