Experiment 2: Understanding Liver Fat Metabolism

Not Applicable

Completed

• Conditions: Fatty Acid Oxidation
• Interventions: Other: Dietary fatty acid partitioning

• Registration Number: NCT03587753
• Lead Sponsor: University of Oxford

Brief Summary

There is a largely unsubstantiated thought in the literature that dietary unsaturated enter oxidation pathways preferentially compared to saturated fatty acids. This study is undertaking a direct comparison of the oxidation of dietary saturated and unsaturated fatty acids.

Detailed Description

Provided subjects meet the inclusion criteria, then at least one week after the screening visit volunteers will then undertake the first two 'study days'. There will be two study days on separate occasions that will be up to 3 weeks apart. Each study day will be approximately 7 hours in duration. On each study day participants will be given a standardised 'test' meal that contains specially labelled fat molecules (stable-isotope tracer) and then repeated breath and blood samples will be taken over the course of the next 6 hours. The test meal consumed on each 'study day' will be identical in composition with only the specially labelled fat molecules being different (either unsaturated or saturated fat) and these will be given in a random order.

Study Timeline

• Study Start: 2018-05-01
• Study First Submitted: 2018-06-20
• Study First Submitted QC: 2018-07-03
• Study First Posted: 2018-07-16
• Primary Completion: 2021-01-01
• Study Completion: 2021-01-01
• Status Verified: 2022-06
• Last Update Submitted: 2022-06-06
• Last Update Posted: 2022-06-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

Participant is willing and able to give informed consent for participation in the study.

Male or Female, aged >18 and <65 years BMI >19 <35kg/m2 No medical condition or relevant drug therapy known to affect liver metabolism

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Exclusion Criteria

• • Age <18 or >65 years

  • Body mass index <19 or >35kg/m2
  • A blood haemoglobin <120mg/dL
  • Any metabolic condition or relevant drug therapy
  • Smoking
  • History of alcoholism or a greater than recommended alcohol intake
  • Pregnant or nursing mothers
  • Women prescribed hormone replacement therapy (HRT) or who have used this within the last 12 months
  • History of severe claustrophobia
  • Presence of metallic implants, pacemaker
  • Haemorrhagic disorders
  • Anticoagulant treatment

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Unsaturated	Dietary fatty acid partitioning	A test meal containing a unsaturated fatty acid labelled with 13C to measure hepatic fatty acid partitioning.
Saturated	Dietary fatty acid partitioning	A test meal containing a saturated fatty acid labelled with 13C to measure hepatic fatty acid partitioning

Primary Outcome Measures

Name	Time	Method
Dietary fatty acid partitioning	6 hours	The appearance of the 13Clabelled fatty acid in plasma triglycerides

Trial Locations

Locations (1)

Oxford Centre for Diabetes, Endocrinology and Metabolism; 🇬🇧 Oxford, United Kingdom