Relative Energy Deficiency in Females and Males Rowers - Observational Study
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Leptin to measure long-term energy balance
研究概览
简要总结
This study aims to learn about relative energy deficiency in male and female rowers at the end of the direct sport preparation phase of the annual training cycle. The main questions:
- How will energy intake influence leptin, cortisol levels, mood, gut discomfort, and permeability? The observational study involves male and female rowers from the Polish rowing team.
Participants will perform 2000 meter ergometer test and 6000 ergometer test in annual training cycle. Researchers will compare outcomes from both tests.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 23 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Membership in Polish Rowing Team,
- •minimum five years of training,
- •total training time minimum of 240 minutes,
- •filling out a food diary,
- •finishing 2000-meter ergometer test.
排除标准
- •Probiotics within the last three months,
- •prebiotics within the last three months
- •antibiotic therapy within the last three months,
- •dietary regime,
- •gastrointestinal diseases.
结局指标
主要结局
Leptin to measure long-term energy balance
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period
Leptin concentration measured in blood \[ng/ml\] using commercially availableenzyme-linked immunosorbent assays
Claudin to measure tight junction leakage
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period
Claudin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Cortisol to measure stress
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period
Stress hormone concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
REST-Q sport
时间窗: day before the test
Recovery-Stress questionnaire for Athletes
-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period
I-FABP concentration measured in blood \[ng/ml\] using commercially availableenzyme-linked immunosorbent assays
sCD14 multifunctional lipopolysaccharide receptor
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period
sCD14 concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays
POMS
时间窗: day before the test
Profile of Mood States
次要结局
- fat(Time Frame: whole day (24hours) before test, in the morning before the test)
- carbohydrate(Time Frame: whole day (24hours) before test, in the morning before the test)
- energy(Time Frame: whole day (24hours) before test, in the morning before the test)
- fiber(Time Frame: whole day (24hours) before test, in the morning before the test)
- Body fat(in the morning before the test up to 1 hour after getting up)
- Total body water(in the morning before the test up to 1 hour after getting up)
- protein(Time Frame: whole day (24hours) before test, in the morning before the test)
- Body mass(in the morning before the test up to 1 hour after getting up)
- Self reported scale of gastrointestinal symptoms e.g.burping, heartburn, flatulence, abdominal pain, the urge to reiterate, vomiting, diarrhoea, nausea, dizziness, and stitch(up to 1 hours after the test)
- Lean body mass(in the morning before the test up to 1 hour after getting up)
- Height(in the morning before the test up to 1 hour after getting up)