Assessment of Saliva Adseverin,1-Alpha Defensin and sRANKL (Soluble RANKL) Levels In Individuals With Different Periodontal Diseases
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- The total amount of 1-Alpha Defensin in saliva
研究概览
简要总结
: The aim of this study is; detection of Adseverin,1-alpha Defensin,sRANKL(soluble RANKL) levels in saliva samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of saliva were obtained from 60 systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine Adseverin,1-alpha Defensin,sRANKL(soluble RANKL) levels in the biological samples.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 20 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.) At least twenty permanent teeth in the mouth Non-smoker No medication for continuous use Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- •Not in pregnancy or lactation period. For the periodontitis group that has not received periodontal treatment in the last 6 months For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
排除标准
- •Any oral or systemic disease Regularly using a systemic medication During pregnancy or lactation Received periodontal treatment within the last 6 months. Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months Smokers are not included in the study
研究组 & 干预措施
Gingivitis
Full-mouth clinical periodontal measurements recorded and saliva obtained.
干预措施: Saliva obtaining (Diagnostic Test)
Healthy Periodontium
Full-mouth clinical periodontal measurements recorded and saliva obtain
干预措施: Saliva obtaining (Diagnostic Test)
Periodontitis
Full-mouth clinical periodontal measurements recorded and saliva obtained.
干预措施: Saliva obtaining (Diagnostic Test)
结局指标
主要结局
The total amount of 1-Alpha Defensin in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of 1-Alpha Defensin in saliva
The total amount of Adseverin in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit]
The total amount of Adseverin in saliva
The total amount of sRANKL in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of soluble RANKL in saliva
次要结局
未报告次要终点
研究者
Ayşe Efe
Research Assistant at Periodontology
Izmir Katip Celebi University