Assessment of Saliva Galectin-8, Galectin-9 and RANKL Levels in Individuals With Different Periodontal Diseases
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- The total amount of Galectin-8 in saliva
研究概览
简要总结
The aim of this study is; detection of Galectin-8, Galectin-9 and RANKL levels in saliva samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of saliva were obtained from 60 systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine Galectin-8, Galectin-9 and RANKL levels in the biological samples.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.)
- •At least twenty permanent teeth in the mouth
- •Non-smoker
- •No medication for continuous use
- •Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- •Not in pregnancy or lactation period.
- •For the periodontitis group that has not received periodontal treatment in the last 6 months
- •For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss
- •For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss
- •For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
排除标准
- •Any oral or systemic disease
- •Regularly using a systemic medication
- •During pregnancy or lactation
- •Received periodontal treatment within the last 6 months.
- •Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
- •Smokers are not included in the study
结局指标
主要结局
The total amount of Galectin-8 in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of Galectin-8 in saliva
The total amount of Galectin-9 in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of Galectin-9 in saliva
The total amount of RANKL in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of Receptor activator of nuclear factor kappaB ligand (RANKL) in saliva
次要结局
未报告次要终点
研究者
Kübra Aydın
Research Assistant
Izmir Katip Celebi University