Assessment of Saliva Galectin-7, Galectin-10 and MMP-9 Levels In Individuals With Different Periodontal Diseases
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- The total amount of Gal-10 in saliva
研究概览
简要总结
The aim of this study is; detection of Galectin-10, Galectin-7, MMP-9 levels in saliva samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of saliva were obtained from 60 systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine Galectin-10, Galectin-7 and MMP-9 levels in the biological samples.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.)
- •At least twenty permanent teeth in the mouth
- •Non-smoker
- •No medication for continuous use
- •Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- •Not in pregnancy or lactation period.
- •For the periodontitis group that has not received periodontal treatment in the last 6 months
- •For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss
- •For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss
- •For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
排除标准
- •Any oral or systemic disease
- •Regularly using a systemic medication
- •During pregnancy or lactation
- •Received periodontal treatment within the last 6 months.
- •Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
- •Smokers are not included in the study
结局指标
主要结局
The total amount of Gal-10 in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of Galectin-10 in saliva
The total amount of Gal-7 in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of Galectin-7 in saliva
The total amount of MMP-9 in saliva
时间窗: 24 hours after taking the clinical measurements at the first visit
The total amount of Matrix metalloproteinase 9 in saliva
次要结局
未报告次要终点
研究者
Ceren Koksal
Research Assistant at Periodontology
Izmir Katip Celebi University