Gene Expression and Genome Wide Association Study on remission of asthma
Completed
- Conditions
- asthmahyperresponsivenes10006436
- Registration Number
- NL-OMON40066
- Lead Sponsor
- niversitair Medisch Centrum Groningen
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 277
Inclusion Criteria
Roorda cohort and the DAG cohort (asthma)
1. Age 18 years or older
2. Previous diagnosis of asthma
3. Bronchial hyperresponsiveness in childhood (Roorda Cohort)
4. In clinical or complete remission of asthma (DAG cohort)
NORM study (healthy)
1. Age 18 years or older
2. FEV1 pre bronchodilator > 80% predicted
3. No bronchial hyperresponsiveness; PC20 metacholinebromide >32 mg/ml
4. Never diagnosed with asthma
Exclusion Criteria
For all cohorts
1. Presence of serious concomitant diseases (Cancer, Cardiovascular disorders)
2. Pregnancy
Additional exclusion criterium for metacholine challenge testing:
3. FEV1 below 1.2 L
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The main outcome parameter is the expression level of mRNA. These expression<br /><br>levels will be compared between patients in complete asthma remission, clinical<br /><br>asthma remission, no asthma remission and healthy controls.</p><br>
- Secondary Outcome Measures
Name Time Method <p>1. The genetic variation at DNA-level will be used to perform a GWAs study on<br /><br>the presence of asthma remission.<br /><br>2. Association of IL1RL1 gene variation, with IL1RL1 T lymphocyte expression<br /><br>with and without IL-33 stimulation.<br /><br>3. Investigate the differences in DNA-methylation between asthmatics and<br /><br>patients in clinical or complete remission<br /><br>4. Cultivate nasal epithelial cells for hypothesis-testing in functional<br /><br>studies based on results of the genetic expression and association analyses.<br /><br>5. To compare the small airway parameters Scond and Sacin between patients<br /><br>in complete remission of asthma, clinical remission of asthma and patients with<br /><br>current asthma. </p><br>