Adenosylmethionine Metabolism in Human Inflammation

• Conditions: Arthritis; Chronic Inflammation

• Registration Number: NCT02520206
• Lead Sponsor: National Chung Hsing University

Brief Summary

The investigators propose to conduct a translational study on the regulation of S-adenosylmethionine synthesis and cellular methylation reactions during chronic inflammation. Development of in vitro cell models may reveal the regulatory mechanisms by which specific inflammatory mediators cause metabolic changes and alter DNA methylation status. Metabolic and pharmacological studies in the in vivo models will enable us to better understand the regulation of inter-organ homeostasis of S-adenosyl methionine and help identify tissue specific biomarkers for methylation and epigenetic modifications in different stage of chronic inflammation. The clinical study in human subjects will help distinguish the impacts of autoimmune rheumatic disease, degenerated joint disease, or specific medication use on significant clinical and biochemical markers in folate and vitamin B6 metabolic pathways.The Investigators hope the present study can identify specific clinical markers for potential epigenetic changes in patients suffering from chronic inflammation, which will contribute to better clinical management of these diseases in humans.

Detailed Description

The significance of epigenetic alterations in autoimmune rheumatic diseases and degenerated joint diseases has drawn great attention among clinicians and researchers. Aberrant methylation status has been demonstrated in human chronic inflammation yet more efforts have focused on global and sequence-specific hypomethylation and overexpression of specific genes. Few studies investigated the regulation of S-adenosylmethionine homeostasis and regulation during inflammation. At present the relevance and regulation of the complex epigenetic profiles and their modifications among different tissues and organs during inflammation remain largely unknown.

Study Timeline

• Study Start: 2011-01
• Primary Completion: 2014-07
• Study First Submitted: 2015-07-28
• Status Verified: 2015-08
• Study Completion: 2015-08
• Study First Submitted QC: 2015-08-06
• Last Update Submitted: 2015-08-06
• Study First Posted: 2015-08-11
• Last Update Posted: 2015-08-11

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 250

Inclusion Criteria

• > 18 years

Exclusion Criteria

• pregnancy,
• anemia (hemoglobin 10 mg/dL or lower),
• thrombocytopenia (platelet count below 50,000 cells/μL),
• abnormal serum hepatic transaminase (aspartate aminotransferase or alanine aminotransferase above 50 IU/L),
• diabetes or cancer

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
s-adenosylmethionine	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites
homocysteine	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites
folate	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites
vitamin B6	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites

Secondary Outcome Measures

Name	Time	Method
blood amino acid profile (serine, glycine, methionine,cysteine, cystathionine, dimethylglycine)	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites
polymorphisms in one carbon metabolism enzymes in PBMCs	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses
gene expression of target enzymes in PBMCs	Blood were collected at admission.Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses
enzyme activities of S-adenosylmethionine synthase in RBC	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites
vitamin B6 metabolic enzyme in RBC	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites