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Nut Intake At Night: Effect on Postprandial Glycaemia

Not Applicable
Withdrawn
Conditions
Insulin Resistance
Postprandial Hyperglycemia
Interventions
Other: Nut meal
Other: Control meal
Registration Number
NCT04965896
Lead Sponsor
Monash University
Brief Summary

There is some evidence to suggest that the timing of a meal intake directly impacts postprandial insulin and glucose responses, with meals consumed later during the day being more metabolically detrimental that the same meals consumed during the day. This information is particularly pertinent to the 16% of people employed in shift-work professions in Australia who have little choice but to eat during the late evening and overnight. The purpose of this study is to compare two effect of different meals or snacks (control vs test meal) on blood glucose and insulin at night time in healthy adults. This study will enable to develop suitable meals to consume at night time that can reduce the higher glucose and insulin responses that are a consequence of eating late into the night.

Detailed Description

Eating in the morning results in a more effective clearance of blood sugar (glucose). However, some people, such as shift workers, have no choice but to eat at night. Previous studies have shown that consuming protein at night can be effective at lowering blood sugar. The investigators here propose that nuts would be a healthy snack to eat at night time as they are a good source of protein and may help to reduce the high levels of blood glucose observed by eating at night time.

The aim of this cross-over study is to compare the effect of a snack high in nuts compared with a control snack similar in macronutrients (cheese and crackers) on postprandial blood glucose and insulin in healthy participants after consumption of a meal known to raise blood sugar (white rice). The investigators hypothesise that compared with a standard snack, a snack containing nuts will show a lower postprandial glucose and insulin response.

The participants will complete an initial screening questionnaire to check eligibility. Eligible participants will attend the research facilities after 5h fasting at night (from 18.30pm-22.00pm) on two occasions, when they will receive plain white rice (75g of available carbohydrate) alongside either 30 g of mixed nuts (test meal) or a snack with cheese and crackers (control meal) that is equivalent in energy and macronutrient content. During the visits, a finger prick fasting glucose sample will be collected. Repeated finger prick samples will be collected at 15, 30, 45, 60, 90, 120, 150 and 180 minutes after the meal intake. These samples will be used for glucose and insulin measurement. Postprandial glucose and insulin iAUC will be calculated. Differences in glucose and insulin concentrations and iAUC as well as glucose time to peak will be compared between the two treatment conditions.

Recruitment & Eligibility

Status
WITHDRAWN
Sex
All
Target Recruitment
12
Inclusion Criteria
  • 18 to 50 years of age
  • Waist circumference of <94cm and <80cm for Caucasian males and females, respectively
  • Waist circumference of <90cm and <80 cm for Asian males and females, respectively
  • Available to attend two testing sessions at the research facility
Exclusion Criteria
  • Diagnosed with type 2 diabetes or taking anti-diabetic medication (oral hypoglycaemic agents)
  • Impaired fasting glucose (≥ 7 mmol/L)
  • Gastrointestinal conditions that may affect glycaemic response
  • Serious health conditions that may affect participation e.g. liver or thyroid dysfunction, recent major surgery
  • Women planning pregnancy, pregnant or lactating.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Nut mealNut mealParticipants will receive plain white rice (75g of available carbohydrate) alongside a portion of 30g of nuts. The meal is similar in energy and macronutrients to the control meal.
Control mealControl mealParticipants will receive plain white rice (75g of available carbohydrate) alongside a snack with crackers and cheese. The meal is similar in energy and macronutrients to the test meal.
Primary Outcome Measures
NameTimeMethod
Postprandial plasma insulin iAUCThree hour glucose iAUC will be calculated at seven time points (0, 30, 60, 90, 120, 150 and 180 mins) after beginning consumption of the test meal.

Difference in postprandial plasma insulin iAUC

Postprandial plasma glucose area under the curve (iAUC)Three hour glucose iAUC will be calculated at nine time points (0, 15, 30, 45, 60, 90, 120, 150 and 180 mins) after beginning consumption of the test meal.

Difference in postprandial plasma glucose iAUC

Secondary Outcome Measures
NameTimeMethod
Time to peak glucoseThree hours

Difference in time to peak glucose

Postprandial insulin concentrationInsulin concentration will be measured in finger prick blood samples at seven time points (0, 30, 60, 90, 120, 150 and 180 mins) after beginning consumption of the test meal.

Difference in postprandial insulin concentration

Postprandial glucose concentrationGlucose concentration will be measured at nine time points (0, 15, 30, 45, 60, 90, 120, 150 and 180 mins) after beginning consumption of the test meal.

Difference in postprandial glucose concentration

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