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Time Lag for Oocyte Denudation: A Better Choice for ICSI Outcome?!

Completed
Conditions
Infertility, Female
Registration Number
NCT04185623
Lead Sponsor
Ahmed Saad
Brief Summary

This study aiming to investigate if difference in the timing of cumulus removal post retrieval would have any critical impact on fertilization and embryonic development.

Detailed Description

Patients under 37 year old who were treated with controlled ovarian stimulation for ICSI were randomly divided into 2 groups; Group A: Oocytes were denudated immediately after oocyte retrieval , Group B: Oocytes were denudated 2 hours after retrieval and both groups were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
300
Inclusion Criteria
  • Women 24-37 years old
  • With good ovarian reserve
  • Induction using Long agonist protocol
  • unexplained infertility
Exclusion Criteria
  • severe male factor
  • Testicular samples for ICSI
  • poor ovarian reserve
  • no of oocytes collected less than 4

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Day 5, good quality blastocyst120 hour post ovum pickup

Assessment were done according to gardener classification

Secondary Outcome Measures
NameTimeMethod
clinical pregnancy6 weeks from the last normal menstrual period

Assessment was done by observation of a gestational sac at 6 wks

Trial Locations

Locations (1)

Hawaa Fertility Center

🇪🇬

Banhā, Qalyubiya, Egypt

Hawaa Fertility Center
🇪🇬Banhā, Qalyubiya, Egypt

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