Effects of Oral Nitrate Supplementation on Vascular and Pulmonary Oxidative Stress Markers Following Traffic Pollution Exposure
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Change in plasma nitrite concentration
研究概览
简要总结
The study hypothesis is that drinking different juices will affect the body's responses to air pollution. Subjects will be exposed to air pollution during a 2-hour car ride on the NJ (New Jersey) Turnpike. Each subject will be asked to do this twice. Before one car ride, the subject will be asked to drink orange juice. Before the other car ride, the subject will be asked to drink beet juice. Samples of blood and exhaled breath will be collected before, immediately after, and 24 hours after each car ride. Levels of nitrites/nitrates will be measured in the blood and breath.
详细描述
Subjects will participate in two exposure sessions. The air pollution exposure consists of a 2-hour car ride along the NJ Turnpike. Samples will be collected at 3 time points for each exposure session: pre-exposure, immediately post-exposure, and 24 hours post-exposure. Blood samples will be collected immediately before and after a 5-minute occlusion at each time point. The subject will also be asked to provide samples of exhaled breath and complete symptom and stress questionnaires at each time point.
Blood samples will be analyzed for serum nitrite. Exhaled breath samples will be analyzed for nitrates and nitrites.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Must live within 20 miles of Piscataway, NJ
排除标准
- •• Are a current smoker
- •Are pregnant or breastfeeding
- •Are HIV-positive
- •Have an active medical condition such as high blood pressure, asthma, diabetes or other serious disease
- •Have a heart condition such as atrial flutter, atrial fibrillation or a pacemaker
- •Are unable to ride comfortably in a car for 2 hours
- •Travel to the EOHSI (Environmental and Occupational Health Sciences Institute) Clinic Center on major highways for more than 3 miles (the Garden State Parkway, the New Jersey Turnpike, Routes 287, 1, 18, or 9).
研究组 & 干预措施
Orange Beet
Orange juice will be given before the first car ride and beet juice will be given before the second car ride.
干预措施: Beet juice (Dietary Supplement)
Beet Orange
Beet juice will be given prior to the first car ride and orange juice will be given prior to the second car ride.
干预措施: Beet juice (Dietary Supplement)
Beet Orange
Beet juice will be given prior to the first car ride and orange juice will be given prior to the second car ride.
干预措施: Orange juice (Dietary Supplement)
Orange Beet
Orange juice will be given before the first car ride and beet juice will be given before the second car ride.
干预措施: Orange juice (Dietary Supplement)
结局指标
主要结局
Change in plasma nitrite concentration
时间窗: Prior to the car ride, immediately after the 2-hour car ride, and 24 hours later
Change in plasma nitrite concentration pre- and post-occlusion
次要结局
- Change in nitrite/nitrate concentration in exhaled breath(Prior to the car ride, immediately after the 2 hour car ride and 24 hours later)
研究者
Howard M. Kipen, MD, MPH
Professor and Department Chair
Rutgers, The State University of New Jersey