Preclinical Brain Tumor Models

Recruiting

• Conditions: Brain Metastases; Diffuse Gliomas
• Interventions: Other: Fresh tumor sampling

• Registration Number: NCT06045065
• Lead Sponsor: Assistance Publique Hopitaux De Marseille

Brief Summary

Background: Primary or secondary brain tumors (metastases) remain associated with a very poor prognosis linked to significant therapeutic resistance. Thus, glioblastoma, which is the most common and aggressive primary brain tumor in adults, is associated with inevitable relapses within 7 to 10 months and median survival of approximately 12 to 14 months. At the same time, brain metastases are increasingly increasing following better systemic control of other metastatic sites and improvement in detection methods. However, they remain resistant to the latest therapeutic innovations such as immunotherapies or targeted therapies.

In this context, innovative strategies are necessary to identify new therapeutic targets and implement new treatments to overcome resistance phenomena in the clinic.

Objective: Our goal will be to generate tumoroids and stem-like cell lines (PDX) from patient tumor samples.

Methods: We will establish tumor tumoroids and stem cell lines from patient samples. These preclinical models will allow us to test a large number of drugs, quickly and efficiently thanks to models as close as possible to patient tumors and limiting the use of animal models (3R).

Overall, this project should enable major advances in the treatment of glioblastoma and brain metastases and enable the rapid testing of new molecules in clinical trials thanks to the homology of our models with our patients' diseases.

Detailed Description

Not available

Study Timeline

• Study Start: 2022-01-02
• Status Verified: 2023-09
• Study First Submitted: 2023-09-13
• Study First Submitted QC: 2023-09-13
• Last Update Submitted: 2023-09-13
• Study First Posted: 2023-09-21
• Last Update Posted: 2023-09-21
• Primary Completion: 2024-01
• Study Completion: 2025-01

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

• Adult patients
• Brain tumor neuro-imaging
• Eligible for neurosurgery

Exclusion Criteria

• unresectable tumor

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
patients with primitive tumor	Fresh tumor sampling	Patient samples will be collected during routine care neurosurgical resection.
patients with brain metastasis	Fresh tumor sampling	Patient samples will be collected during routine care neurosurgical resection.

Primary Outcome Measures

Name	Time	Method
Tumoroid and stem cell line generation	4 weeks after generation	The generation of tumoroids and stem cell lines will be attested at 4 weeks after initiation of their culture.; Tumoroids: generation of round and viable structures Stem cell lines: generation of neurospheres

Trial Locations

Locations (1)

Assistance publique hopitaux de marseille; 🇫🇷 Marseille, France