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A First-in-human Single Ascending Dose/Multiple Ascending Dose Study of ENN0403 in Healthy Subjects

Phase 1
Completed
Conditions
Healthy
Interventions
Drug: ENN0403 4mg
Drug: ENN0403 10mg
Drug: ENN0403 1mg
Drug: ENN0403 12mg QD X 14 Days
Drug: ENN0403 20mg QD X 14 Days
Drug: ENN0403 20mg
Drug: ENN0403 30mg
Drug: ENN0403 20mg (Fed)
Drug: ENN0403 6mg QD X 14 Days
Drug: Placebo
Registration Number
NCT05506540
Lead Sponsor
EnnovaBio Australia Pharmaceuticals Pty Ltd
Brief Summary

This is a FIH, randomized, double- blind, placebo-controlled, dose -escalation study to investigate the safety, tolerability, PK, and PD of ENN0403 after single and multiple oral dose administration in healthy adult subjects. The study will include 2 parts which will proceed in a parallel staggered manner: Part A, a single ascending dose (SAD) study and Part B, a multiple ascending dose (MAD) study.

Approximately 80 healthy adult subjects will be enrolled at a single site in Australia, in up to 6 cohorts in Part A (SAD study), including a Food Effect (FE) study, and up to 4 cohorts in Part B (MAD study). Part A is for the single dose use of IP, while Part B is once daily use for 14 consecutive days. Each cohort will include 8 subjects (6 receiving ENN0403 and 2 receiving placebo). Each subject will be enrolled in only 1 cohort and receive only one dose regimen in this study.

Dosing will be escalated in a sequential fashion, contingent on a review of safety, tolerability, and available PK data of the previous dose level by a Safety Review Committee (SRC). The proposed dose levels/ dosing frequency of ENN0403 may be adjusted over the course of the whole study and cohorts may be added or removed depending on the emerging safety, tolerability, and available PK data.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
69
Inclusion Criteria
  • Capable of giving signed informed consent.
  • 18 to 55 years old (inclusive).
  • BMI of 18 to 30 kg/m2 (inclusive); body weight >50 to <100 kg for male subjects or >45 to <100 kg for female subjects.
  • Computerized (12-lead) ECG recording without signs of clinically relevant pathology or showing no clinically relevant deviations as judged by the PI.
  • Test negative for COVID-19.
  • Test negative for HBsAg, anti-HBc, anti-hepatitis C virus (HCV) antibodies, anti-human immuno deficiencyvirus (HIV) 1 and 2 antibodies, and tuberculosis.
  • Have a negative urine drug screen and a negative alcohol breath test.
  • Nonsmoker or occasional smoker and willingness to refrain from smoking during study.
  • Ability and willingness to abstain from alcohol during study.
  • not pregnant, not breastfeeding; apply contraception methods for child-bearing potential subjects.
Exclusion Criteria
  • History of clinically significant hematological, renal, endocrine, pulmonary, gastrointestinal,cardiovascular, hepatic, psychiatric, neurologic, or allergic disease in the opinion of the Investigator within 12 months prior to Screening.
  • Any disease or take any medication that affects IP absorption, distribution, metabolism, and excretion.3. Family history of sudden death or of congenital prolongation of the QTc interval or known congenital prolongation of the QTc interval or any clinical condition known to prolong the QTc interval.
  • Presence of malignancy including hematological malignancies. Subjects with a history of basal cell or squamous cell carcinoma that has been treated with no evidence of recurrence within 3 years of Screening will be allowed for inclusion, as judged by the Investigator.
  • Any current active infections, including localized infections, or any recent history (within 1 week prior to IP administration) of active infections, cough or fever; or a history of recurrent or chronic infections.
  • In the 12-lead ECG assessment, QTcF >450 ms for male subjects or >470 ms for female subjects.7. Estimated glomerular fltration rate <90 mL /min (using the Cockcroft-Gault formula) at Screening.
  • ALT or aspartate aminotransferase>1.5ULN.
  • Have received any live vaccines (bacterial or viral) within 12 weeks prior to Screening or intend to receive a live vaccine during the study period or within 30 days after the last dose of the IP.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
Single Ascending Dose, ENN0403 4 mgENN0403 4mgSingle oral use of ENN0403 at dose level 4 mg, in fasted state.
Single Ascending Dose, ENN0403 10 mgENN0403 10mgSingle oral use of ENN0403 at dose level 10 mg, in fasted state.
Single Ascending Dose, ENN0403 1 mgENN0403 1mgSingle oral use of ENN0403 at dose level 1 mg, in fasted state.
Multiple Ascending Dose, ENN0403 12 mgENN0403 12mg QD X 14 DaysENN0403 capsules for oral administration, 12 mg QD X 14 Days
Multiple Ascending Dose, ENN0403 20 mgENN0403 20mg QD X 14 DaysENN0403 capsules for oral administration, 20 mg QD X 14 Days
Single Ascending Dose, ENN0403 20 mgENN0403 20mgSingle oral use of ENN0403 at dose level 20 mg, in fasted state.
Single Ascending Dose, ENN0403 30 mgENN0403 30mgSingle oral use of ENN0403 at dose level 30 mg, in fasted state.
Single Ascending Dose, ENN0403 20 mg (Fed)ENN0403 20mg (Fed)Single oral use of ENN0403 at dose level 30 mg, after high calorie and high-fat breakfast meal.
Multiple Ascending Dose, ENN0403 6 mgENN0403 6mg QD X 14 DaysENN0403 capsules for oral administration, 6 mg QD X 14 Days
Single/Multiple Ascending Dose, placebo capsules for oral adminstrationPlaceboPlacebo capsules for oral administration
Primary Outcome Measures
NameTimeMethod
Number of participants with treatment emergent adverse events (TEAE) following ENN0403 administrationFrom first dose of ENN0403 administration till 7 days after last dose of ENN0403 administration.
Secondary Outcome Measures
NameTimeMethod
Maximum Plasma Concentration [Cmax]Single Ascending Dose (SAD) part: up to Day 4, 72 hours post dose; Multiple Ascending Dose (MAD) part: up to Day 17, 72 hours post last dose

Cmax is the maximum measured concentration of ENN0403 in plasma after oral administration

Area Under the Curve [AUC]Single Ascending Dose (SAD) part: up to Day 4, 72 hours post dose; Multiple Ascending Dose (MAD) part: up to Day 17, 72 hours post last dose

AUC is the area under the concentration-time curve of ENN0403 in plasma after oral administration

Trial Locations

Locations (1)

CMAX Clinical Research Pty Ltd

🇦🇺

Adelaide, Australia

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