Investigation of Microbiome-Derived Metabolite Production in Response to a Mediterranean Diet
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 21
- 试验地点
- 1
- 主要终点
- Microbiome-derived metabolite profiling
研究概览
简要总结
This project proposes to characterise the microbiome-derived metabolites produced upon ingestion of bioactive precursor-rich meals by healthy volunteers in order to investigate the relationship between gut microbiome composition and the nature of dietary metabolites production.
详细描述
There is a large inter-individual variability in gut microbiome-derived metabolites production upon ingestion of similar meals. Since many of these microbiome-derived metabolites have enteric and systemic biological effects, these variabilities could in part account for the different responses to meals and diets.
This project thus aims to improve the understanding the relationship between gut microbiome composition and the nature of dietary metabolites produced from similar bioactive precursor-rich meals.
The investigators therefore propose to characterise the circulating microbiome-derived metabolite profiles in healthy individuals fed the same 2-day precursor-rich Mediterranean diet prior to and after a 2-week of habitual Canadian diet feeding.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Men and women aged between 18 and 35 years
- •Body mass index between 18.5 and 30 kg/m2
- •General good health
- •Computer and Internet access
- •Basic understanding of written French
排除标准
- •Intestinal pathologies including inflammatory bowel disease and gastrointestinal cancers
- •Pregnant and/or breast-feeding women
- •Alcohol consumption greater than 15 portions for men and 10 for women weekly
- •Active tobacco usage
- •Consumption of dietary supplements (e.g. multivitamins, omega-3, probiotics)
- •Completion of a course of antibiotics in the past 3 months
- •Regular use of anti-inflammatory drugs, or use in the past 3 months
- •Important weight change (+/- 5 kg) in the past 6 months
- •Allergies or food intolerances to ingredients present in trial diets
研究组 & 干预措施
Single
All participating subjects will undergo two, 2-day Mediterranean diet feeding periods separated by a 14-day period of Canadian diet
干预措施: Mediterranean diet (Other)
结局指标
主要结局
Microbiome-derived metabolite profiling
时间窗: 19 days
Plasma and urine metabolomics profiling of gut-microbiome derived metabolites (e.g., branched-chain amino acids \[BCAA\], short chain fatty acids \[SCFA\], trimethylamine \[TMA\], amino acids- and polyphenol-metabolites) will also performed at the same time points.
Gut microbiome characterisation
时间窗: 19 days
Fecal microbiome composition will be characterise by rRNA 16S sequencing prior to and following both 2-day Mediterranean diet periods. Relevant indexes of microbiome diversity (e.g. Shannon beta-diversity), ratios between meaningful bacterial markers of gut dysbiosis (e.g. Firmicutes:Bacteroidetes) and specific microbiome differences in bacterial Families, Genera or species between study periods and individuals.
次要结局
- Endocannabinoid interaction with interindividual microbiome composition(19 days)