High intensity interval training and skeletal muscle insulin sensitivity: unrevealling health effects and underlying mechanisms

Completed

Conditions: Insulin resistance
obesity
10018424

Registration Number: NL-OMON55499

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 17

Inclusion Criteria

Age 45-75 years old
Overweight to obese (BMI *27 kg/m2)
Sedentary: subjects do not perform any regular physical activity weekly (<3
times per week, <150 min/week).

Exclusion Criteria

Unstable body weight (weight gain or loss > 3 kg in the past three months)
Previously diagnosed with type 2 diabetes
Use of beta-blockers

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1. * Non-oxidative glucose disposal (µmol/kg/min) by<br /><br>hypersinsulinemic-euglycemic clamp combined with indirect calorimetry: NOGD is<br /><br>reported as the difference between the rate of glucose disposal (Rd) and<br /><br>carbohydrate oxidation under insulin-stimulated condition.<br /><br></p><br>

Secondary Outcome Measures

Name	Time	Method
<p>1. - Skeletal muscle glycogen content (mmol/kg protein) and regulatory enzymes<br /><br>activity<br /><br><br /><br>2. - Skeletal muscle insulin sensitivity (Rd: insulin-stimulated basal<br /><br>glucose disposal, µmol/kg/min)<br /><br><br /><br>3. - 24 hours glycaemic profile (Mean glucose concentration mmol/L, net<br /><br>incremental AUC, frequency and duration of hyperglycaemia)<br /><br><br /><br>4. - Skeletal muscle mitochondrial function (PCr recovery time in sec.)<br /><br><br /><br>5. - Metabolic flexibility during exercise (*RER)<br /><br><br /><br>6. - Ectopic fat accumulation in the liver (%)<br /><br><br /><br>7. - Maximal Acetylcarnitine formation in the upper-leg at resting and after<br /><br>exercise (mmol/kg wet weight).</p><br>