The Effects of Hydration on Gut Health and Thinking

Not Applicable

Completed

• Conditions: Cognitive Change; Dehydration; Gastrointestinal Microbiota
• Interventions: Dietary Supplement: Water Intake

• Registration Number: NCT05315531
• Lead Sponsor: University of Illinois at Urbana-Champaign

Brief Summary

The central hypothesis is that improving hydration through increased water consumption will change the relative abundance of mucolytic bacteria found in the stool. Therefore the specific aims are 1) to quantify intervention effects on fecal microbiota relative abundance and plasma lipopolysaccharide binding protein, 2) observe the effects of the intervention on bowel frequency and signs/symptoms of gastrointestinal stress, and 3) to investigate relations between executive function and hydration status.

Detailed Description

A single arm 3-week hydration intervention will be employed where participants increase their water consumption to 2 (F) or 2.5(M) liters per day which is approximately 70% of the AI for daily water consumption. Pre-test and follow-up measures of fecal microbiota, urinary hydration status, cognitive function, circulating markers, and dietary intake will be assessed at baseline and at 3-week follow up via laboratory visits.

Study Timeline

• Study Start: 2021-08-23
• Study First Submitted: 2021-10-01
• Study First Submitted QC: 2022-04-06
• Study First Posted: 2022-04-07
• Primary Completion: 2022-07-29
• Study Completion: 2022-07-29
• Status Verified: 2024-01
• Last Update Submitted: 2024-01-10
• Last Update Posted: 2024-01-11

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 23

Inclusion Criteria

• 19-50 years of age
• 18.5-34.49 kg/m2
• 24-hour UOsm above 500 mOsm/kg
• No antibiotic use over the past 3 months
• Absence of metabolic diseases and use of diuretics
• Agree to maintain typical diet intake (e.g., dietary fiber) patterns during intervention
• Avoid consuming prebiotic and probiotic supplements during study participation
• Not pregnant
• Agree to follow the study protocol

Exclusion Criteria

• <19 or >50 years of age
• <18.5 or >34.49 kg/m2
• 24-hour UOsm <500 mOsm/kg
• Antibiotic use over the past 3 months
• Metabolic diseases and use of diuretics
• Not agree to maintain typical diet intake (e.g., dietary fiber) patterns for the duration of the
• intervention
• Not agree with avoiding consuming prebiotic and probiotic supplements during study participation
• Pregnant
• Not agree to follow study protocol

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Water Intake	Water Intake	3-week intervention period during which articipants will be asked to increase their daily plain water consumption to at least 2.5 L/d of water for males and 2L/d for females.

Primary Outcome Measures

Name	Time	Method
Fecal microbiota relative abundance	3 weeks (baseline vs. follow-up)	changes in the relative abundance of fecal microbiota
Plasma lipopolysaccharide (LPS)	3 weeks (baseline vs. follow-up)	changes in circulating LPS

Secondary Outcome Measures

Name	Time	Method
Attentional accuracy	3 weeks (baseline vs. follow-up)	Accuracy (%) on a computerized flanker task
Attentional Reaction Time	3 weeks (baseline vs. follow-up)	Reaction time (ms) on a computerized flanker task
24hr Urine Osmolality	3 weeks (baseline vs. follow-up)	changes in osmolality (mOsmol/kg) of urine samples
Copeptin	3 weeks (baseline vs. follow-up)	changes in plasma copeptin concentration
Attentional processing speed	3 weeks (baseline vs. follow-up)	P3 event related potential latency (ms) using a computerized flanker task
24hr Urine Specific Gravity	3 weeks (baseline vs. follow-up)	changes in specific gravity (USG) of urine samples

Trial Locations

Locations (1)

University of Illinois at Urbana-Champaign; 🇺🇸 Urbana, Illinois, United States