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A Phase II, Randomized, Double-blind, Placebo-controlled, in Parallel Groups Clinical Trial to Assess the Safety and Efficacy of Dietary Supplementation With Tryptophan to Achieve Weight Loss, and Its Neuropsychological Effects in Adolescents With Obesity

Phase 2
Completed
Conditions
Obesity
Interventions
Dietary Supplement: TRYPTOPHAN
Other: PLACEBO
Registration Number
NCT02612259
Lead Sponsor
Fundació Sant Joan de Déu
Brief Summary

A phase II, randomized, double-blind, placebo-controlled, in parallel groups clinical trial to assess the safety and efficacy of dietary supplementation with tryptophan to achieve weight loss, and its neuropsychological effects in adolescents with obesity.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
44
Inclusion Criteria
  1. Both male and female patients, attending the Pediatric Endocrinology Service on an out-patient basis.
  2. Age from 12 to 17 years, inclusively.
  3. Presence of a BMI > or = to 2SD and < or = to 4SD (standard deviations) for their age and sex (Spanish cross-sectional growth reference tables of 2008)
  4. Patients must have an educational level that permits adequate communication and must agree to cooperate in all the tests and examinations included in the protocol.
  5. Subjects of reproductive potential must use an effective birth control method during the study. Women of reproductive age will be recruited after a negative pregnancy test.
  6. The informed consent of the parents or legal representative and of the young adults is required.
Exclusion Criteria
  1. Obesity secondary to an endocrine disease (hypothyroidism, Cushing?s syndrome, polycystic ovary, hypothalamic syndromes, hypogonadism) or the use of medications such as cortisol.
  2. Concomitant administration of other psychotrophic medication such as antidepressants or anxiolytics.
  3. Patients included in the study must not be taking any vitamins or nutritional supplements or any anti-obesity preparations, including herbal remedies, pharmacy products or homeopathic products.
  4. Patients with a known psychiatric disorder.
  5. Patients treated with any kind of structured psychotherapy regime.
  6. Patients with type 2 DM, arterial hypertension (blood pressure above the 95 percentile for gender and height) or steatotic liver (hypertransaminasemia with echographic image of fatty liver).
  7. Patients with any severe food intolerance, or with a known allergy to any of the substances used in the study.
  8. Patients in treatment with oral hypoglycemiants.
  9. Pregnant or breast-feeding.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
TRYPTOPHANTRYPTOPHANtryptophan 3.5 mg / kg / day divided in 2 doses, one before breakfast and another one before dinner. Oral administration. Total treatment duration for each patient is 6 months.
PLACEBOPLACEBOlactose capsules 3.5 mg / kg / day divided in 2 doses, one before breakfast and another one before dinner. Oral administration. Total treatment duration for each patient is 6 months.
Primary Outcome Measures
NameTimeMethod
Change in weight by comparing the two groupsFrom baseline up to 6 months

to achieve weight loss using Linear Models for Multivariate Outcomes considering as a variables the number of visits, the gender and the group of each patient

Change in systolic and diastolic blood pressureFrom baseline up to 6 months

to achieve improvement of clinical parameters after 6 months of dietary supplementation with tryptophan and determine the difference between the two groups

Change in Body mass index (BMI) defined as wt/ht2 by comparing the two groupsFrom baseline up to 6 months

to achieve weight loss using Linear Models for Multivariate Outcomes considering as a variables the number of visits, the gender and the group of each patient

Change in waist/hip ratio by comparing the two groupsFrom baseline up to 6 months

to achieve weight loss using Linear Models for Multivariate Outcomes considering as a variables the number of visits, the gender and the group of each patient

Change in Body Mass Index Z score (zBMI) by comparing the two groupsFrom baseline up to 6 months

to achieve weight loss using Linear Models for Multivariate Outcomes considering as a variables the number of visits, the gender and the group of each patient

Secondary Outcome Measures
NameTimeMethod
the mean of lipids concentration (mg/dl)From baseline up to 6 months

to achieve improvement of metabolic parameters, the average amount of TSH were measured during visit 1 and visit 7 and then fat levels were compared with the control group. to achieve improvement of metabolic parameters, the average amount of lipids were measured during visit 1 and visit 7 and then fat levels were compared with the control group. The mean of cholesterol both LDL and HDL were considerate for the evaluation.

the amount of Thyroid-stimulating hormone (TSH) concentration (mU/L)From baseline up to 6 months

to evaluate the thyroid profile, the average amount of lipids were measured during visit 1 and visit 7 and then TSH levels were compared with the control group.

the amount of thyroxine T4L concentration (pmol/L)From baseline up to 6 months

to evaluate the thyroid profile, the average amount of T4L were measured during visit 1 and visit 7 and then T4L levels were compared with the control group.

the amount of creatinine levels (mg/dl)From baseline up to 6 months

to evaluate the hepatic profile, the average amount of creatinine were measured during visit 1 and visit 7 and then creatinine levels were compared with the control group.

the amount of Alanine Aminotransferase (ALT) levels (mg/dl)From baseline up to 6 months

to assess the hepatic functionality, the average amount of ALT were measured during visit 1 and visit 7 and then ALT mean levels were compared with the control group.

the amount of hemoglobin (g/dl) levelsFrom baseline up to 6 months

to assess the renal functionality, the average amount of hemoglobin were measured during visit 1 and visit 7 and then hemoglobin mean levels were compared with the control group.

Change in EuroQoL-5 score over 6 months supplement with tryptophanFrom baseline up to 6 months

to achieve improvement in depression and anxiety after tryptophan administration. Evaluation have been made during Visit 1 and vist 7

Change in calorie consumption measuring weight loss (kcal/Kg/days)From baseline up to 6 months

to achieve improvement of metabolic parameters, patient calories intake were measured during visit 1 and visit 7 and then calories levels were compared with the control group.

Change in protein consumptionFrom baseline up to 6 months

to achieve improvement of metabolic parameters, patient protein intake were measured during visit 1 and visit 7 and then proteins levels were compared with the control group.

Total Percent of Calories From ProteinFrom baseline up to 6 months

to achieve improvement of metabolic parameters, patient protein intake were measured during visit 1 and visit 7 and then proteins levels were compared with the control group.

Total fat consumptionFrom baseline up to 6 months

to achieve improvement of metabolic parameters, patient fat intake were measured (grams) during visit 1 and visit 7 and then fat levels were compared with the control group.

Total Percent of Calories From FatFrom baseline up to 6 months

to achieve improvement of metabolic parameters, patient calories intake were measured during visit 1 and visit 7 and then calories levels were compared with the control group.

Total carbohydrates consumptionFrom baseline up to 6 months

to achieve improvement of metabolic parameters, patient carbohydrates intake were measured (grams of carbohydrates) during visit 1 and visit 7 and then carbohydrates levels were compared with the control group.

change in total caloric (Kcal) consumptionFrom baseline up to 6 months

to achieve improvement of metabolic parameters, patient calories intake were measured during visit 1 and visit 7 and then calories levels were compared with the control group.

Change in the Clinical Global Impressions (CGI) Scale score over 6 months supplement with tryptophanFrom baseline up to 6 months

to achieve improvement in depression and anxiety by comparing the tryptophan group with the placebo group

Change in Children's Manifest Anxiety scale (CMAS) score over 6 months supplement with tryptophanFrom baseline up to 6 months

to achieve improvement in depression and anxiety by comparing the tryptophan group with the placebo group

Change in the Eating Attitudes Test (EAT-40) scoreFrom baseline up to 6 months

the EAT-40 test is a widely used self-report questionnaire 40-item standardized self-report measure for the assessment of symptoms and concerns characteristic of eating disorders

Change in State-Trait Depression Scales scoreFrom baseline up to 6 months

The State-Trait Personality Inventory (STPI) is a self-administered questionnaire, consists of eight 10-item subscales: state and trait anxiety, state and trait anger, state and trait curiosity, and state and trait depression. Results from the tryptophan group were compared with the placebo group

Change in Tryptophan plasma level in obese adolescent after 6 months tryptophan administrationFrom baseline up to 6 months

For the safety and tolerance assessment of tryptophan supplementation in obese adolescents

Change in Tryptophan/Large neutral aminoacid (LNNA) ratio in obese adolescent plasma after 6 months of tryptophan administrationFrom baseline up to 6 months

For the safety and tolerance assessment of tryptophan supplementation in obese adolescents

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