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Molecular Diagnostics of Bacterial Infections and Antibiotic Resistance in Blood Samples and Rectal Swabs Using Real Time PCR-ARES PCR Methods

Not Applicable
Recruiting
Conditions
Drug Resistant Bacterial Infection
Interventions
Diagnostic Test: ARES PCR
Registration Number
NCT06308692
Lead Sponsor
IRCCS Sacro Cuore Don Calabria di Negrar
Brief Summary

Experimental study without drug and device, on pseudo-anonymized samples archived at the Tropica Biobank.

The objective is to evaluate the performance for the identification of microorganisms in blood samples and rectal swabs of real time PCR assays for the targets: KPC, VIM, IMP, NDM, Acinetobacter OXA, Oxa48, MCR.

The evaluations will be carried out taking into consideration the data obtained from the diagnostic routine at the DITM.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
100
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Biological samplesARES PCRResidual anonymized samples stored at -80°C at the Tropica Biobank of the DITM and for which diagnostic analysis for germ infection has previously been carried out at the DITM by blood culture and MDR surveillance in routine diagnostic rectal swab will be used.
Primary Outcome Measures
NameTimeMethod
PCR resultsBaseline

Results (positive/negative and microorganism identification) obtained by real time PCR.

Secondary Outcome Measures
NameTimeMethod
Gene mutationsBaseline

Results (resistant/susceptible) obtained by real time PCR in the identification of gene mutations that convey resistance to antibiotics.

Trial Locations

Locations (1)

IRCCS Sacro Cuore Don Calabria hospital

🇮🇹

Negrar, Verona, Italy

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