Protein feeding prior to sleep as a dietary strategy to improve post-exercise recovery and muscle mass in young and elderly me
- Conditions
- 1003700810028302age-related muscle losssarcopenia
- Registration Number
- NL-OMON36991
- Lead Sponsor
- niversiteit Maastricht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 144
Inclusion Criteria
Healthy males
Age between 18 and 35, and 65 and 80
BMI < 30 kg/m2
Exclusion Criteria
• Glucose intolerance
• Milk and/or fat intolerance
• Smoking
• Diagnosed GI tract diseases
• Arthritic conditions
• A history of neuromuscular problems
• Any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications)
• Use of anticoagulants
• Participation in exercise program
• Hypertension, high blood pressure above 140/90 mmHg
• Not participated in any strength training program within the last 3 months
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The main study endpoint is muscle protein synthesis (MPS) rate. In order to<br /><br>determine the MPS, the following parameters<br /><br>will be measured in blood and muscle tissue:<br /><br>• Plasma phenylalanine, leucin and tyrosine concentration (expressed as µmol/L)<br /><br>• Plasma verrijking van L-[ring-2H5]-pheenylalanine, L-[1-13C]-phenylalanine en<br /><br>L-[ring-1-13C]-KIC (expressed as mole percent excess, MPE)<br /><br>• Muscle protein bound enrichment of L-[<br /><br>ring-2H5]-phenylalanine,L-[1-13C]leucine and L-[1-13C]-phenylalanine (expressed<br /><br>as MPE)<br /><br>• L-[1-13C]-phenylalanine enrichment of the muscle free amino acid pool<br /><br>(expressed as<br /><br>MPE)</p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary endpoints include whole-body protein turnover, and protein digestion<br /><br>and absorption kinetics. The following<br /><br>parameters will be calculated:<br /><br>• Total rate of phenylalanine appearance and disappearance (= protein turnover)<br /><br>• Exogenous phenylalanine rate of appearance<br /><br>• Endogenous phenylalanine rate of appearance (=protein breakdown)<br /><br>• Plasma availability of phenylalanine<br /><br>• Plasma availability of amino acids<br /><br>• Plasma glucose concentration<br /><br>• Plasma insulin concentration<br /><br>• Hunger and satiety<br /><br>• Heartrate<br /><br>• Sleep quality</p><br>