DNA Analysis of Tissue Samples From Patients With Head and Neck Cancer

Completed

• Conditions: Head and Neck Cancer

• Registration Number: NCT00898664
• Lead Sponsor: Vanderbilt-Ingram Cancer Center

Brief Summary

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This laboratory study is looking at DNA in tissue samples from patients with head and neck cancer.

Detailed Description

OBJECTIVES:

* Assess common genetic mutations in tumor and germline DNA samples from patients with head and neck squamous cell carcinoma (HNSCC).

* Search for viral DNAs and relate this information to demographics, tumor characteristics, and patient prognosis.

* Search for novel viral sequences and cancer-associated gene mutations in young nonsmoking HNSCC patients and in other distinct head and neck cancers.

* Determine tumor suppressor gene mutations in HNSCC patients and relate this information to gene expression data.

* Validate gene expression information using independent techniques such as real-time PCR on expressed sequences and immunohistochemistry.

OUTLINE: Genetic mutation analysis and gene expression studies are performed on tissue samples from patients with head and neck squamous cell carcinoma. Techniques used include real time-PCR and immunohistochemistry.

Study Timeline

• Study Start: 2006-06
• Primary Completion: 2008-04
• Study Completion: 2008-04
• Study First Submitted: 2009-05-09
• Study First Submitted QC: 2009-05-09
• Study First Posted: 2009-05-12
• Status Verified: 2013-03
• Last Update Submitted: 2013-03-29
• Last Update Posted: 2013-04-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 74

Inclusion Criteria

• Patient with head and neck squamous cell carcinoma (HNSCC) enrolled in the Head and Neck Tissue Repository

  • Sufficient biological material collected to perform study tests

• Historical material available from excess tissues at the Vanderbilt Pathology Department

Exclusion Criteria

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Assess common genetic mutations in tumor and germline DNA samples	Upon collection of final patient data
Viral DNAs related to demographics, tumor characteristics, and patient prognosis	Upon collection of final patient data
Novel viral sequences and cancer-associated gene mutations in young nonsmoking patients with head and neck squamous cell carcinoma and in other distinct head and neck cancers	Upon collection of final patient data
Tumor suppressor gene mutations related to gene expression data	upon collection of final patient data
Validation of gene expression information using independent techniques such as real-time PCR on expressed sequences and immunohistochemistry	upon collection of final patient data