Caries diagnostic using dental plaque and next generation sequencing

Completed

• Conditions: Dental caries; Oral Health

• Registration Number: ISRCTN27083576
• Lead Sponsor: Deutsche Forschungsgemeinschaft

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2017-01-05
• Last Update Posted: 6 February 2017

Recruitment & Eligibility

• Status: Completed
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

Test group caries active:
1. Aged between 18 and 45
2. Minimum 24 teeth
3. Written informed consent
4. Recruitment in the outpatient departments of Saarland and Dresden Universities
5. Sufficient oral hygiene (plaque index < 50%, bleeding index < 15%)
6. Three or more active caries lesions (need of invasive treatment)

Control group caries inactive:
1. Aged between 18 and 45
2. Minimum 24 teeth
3. Written informed consent
4. Recruitment in the outpatient departments of Saarland and Dresden Universities
5. Sufficient oral hygiene (plaque index < 50%, bleeding index < 15%)
6. No active caries lesions, no new fillings within the last 2 years

Exclusion Criteria

1. Smokers
2. Pregnancy
3. Drug use
4. Periodontal disease
5. Other oral diseases except caries
6. Other diseases and conditions

Study & Design

• Study Type: Observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Differences in the biofilms are assessed by amplicon sequencing of the V1 and V2 variable regions of the 16S rRNA gene using MiSeq on samples collected at 2, 4 and 8 hours.

Secondary Outcome Measures

Name	Time	Method
o secondary outcome measures