The Relationship Between ALDH2 and Aortic Dissection

• Conditions: Aortic Dissection

• Registration Number: NCT02630212
• Lead Sponsor: Qilu Hospital of Shandong University

Brief Summary

The purpose of this study is to evaluate the impact of ALDH2 Glu504Lys polymorphism on aortic dissection.

Detailed Description

Not available

Study Timeline

• Study Start: 2015-12
• Study First Submitted: 2015-12-07
• Study First Submitted QC: 2015-12-10
• Study First Posted: 2015-12-15
• Status Verified: 2018-12
• Last Update Submitted: 2019-02-01
• Last Update Posted: 2019-02-05
• Primary Completion: 2019-12
• Study Completion: 2019-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 400

Inclusion Criteria

• Chinese Han people diagnosed with aortic dissection in Qilu Hospital

Exclusion Criteria

• Aortic dissection based on trauma, connective tissue diseases and pregnancy
• Patients with tumor
• Patients with no written consensus

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Number of patients with different ALDH2 genotype	1-30 days after samples collected	DNA was extracted from venous blood;genomic DNA underwent PCR amplification; finally, PCR products were purified and directly sequenced by Invitrogen Corp or Biosune Corp.Then ALDH2 genotype was distinguished depend on a G-to-A missense mutation on exon 12 of ALDH2 gene.

Secondary Outcome Measures

Name	Time	Method
ALDH2 activity	1-30 days after samples collected	Activity of ALDH2 was detected in blood and aortic specimen depend on its reducibility of NAD+
Concentration of inflammatory factors in blood and aortic specimen	1-30 days after samples collected	Concentration of 4-HNE、MMP-2、MMP-9 detected by elisa kit、IHC or western blot in relevant specimen.

Trial Locations

Locations (1)

Qilu Hospital of Shandong University; 🇨🇳 Jinan, Shandong, China