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Alcohol & Metabolic Comorbidities in PLWHA: Evidence Driven Interventions

Not Applicable
Completed
Conditions
Alcohol Use Disorder
HIV/AIDS
Dysglycemia
Interventions
Behavioral: Exercise
Registration Number
NCT03299205
Lead Sponsor
Louisiana State University Health Sciences Center in New Orleans
Brief Summary

Cross-sectional and prospective studies are proposed to test the prediction that a higher proportion of HIV+ individuals with hazardous alcohol drinking with subclinical fasting dysglycemia will present with impaired oral glucose tolerance and dysfunctional metabolic skeletal muscle phenotype. Prospective studies will test the efficacy of an exercise intervention in improving glycemic control. Results will inform larger scale interventions to ameliorate metabolic comorbidities, improve health, quality of life, and possibly decrease hazardous alcohol drinking.

Detailed Description

Adult male and female in care PLWHA with fasting plasma glucose (FPG) \>94 mg/dL and \<125 mg/dL will be recruited. Measures of FPG and HbA1c obtained from overnight fasted subjects. Alcohol use disorder (AUD) will be assessed by AUDIT and time-line follow back questionnaires, coupled with Phosphatidylethanol (PEth) measures. Glucose tolerance will be assessed by oral glucose tolerance test (OGTT). Blood sample collected at the time of the OGTT will be used for determination of circulating levels of adipokines (adiponectin and resistin).

A subset of adult male and female PLWHA +/- AUD with impaired OGTT will be recruited to undergo skeletal muscle (SKM) biopsy (vastus lateralis), 90 min after a defined (calorie and nutrient composition) meal (i.e.,Ensure, Carnation Instant Breakfast). Muscle samples will be used for phenotype characterization (markers of inflammation, insulin signaling, mitochondrial homeostasis) and for myoblast isolation.

Adult male and female PLWHA +/- AUD with impaired OGTT will undergo a moderate intensity aerobic exercise intervention that will include the wearing of an accelerometer (i.e. Fitbit Zip) to monitor daily activity and steps. A SKM biopsy will be performed after completion of the exercise protocol in a subset of subjects to examine the changes in gene expression and myoblast mitochondrial oxidative capacity. We will expand recruitment by enrolling PLWHA +/- AUD based on a FPG between 94-120 mg/dL to participate in the exercise intervention. Clearance for participation in this study will require EKG, completion of a physical activity readiness questionnaire, and medical clearance from a staff clinician. Individuals with significant peripheral neuropathies or the prevalence of significant cardiovascular impairments (i.e. resting cardiac abnormalities or arrhythmias, severe hypertension, etc.) will be excluded from exercise testing or intervention. The 10-week exercise intervention program will consist of aerobic exercise 3 days per week of moderate intensity for 30-45 minutes each session with the goal of achieving 135 minutes per week. The program will begin at low-moderate intensity (40 to 50% of heart rate reserve; HRR) and progress to a higher intensity (50 to 60% HRR) exercise after week 4 of the program. The frequency and duration of exercise sessions will remain constant after week 4 so the absolute dose of exercise will be altered through increasing intensity, i.e. from low-moderate to more vigorous. All exercise will be completed on a treadmill at the Louisiana State University Health Sciences Center (LSUHSC) Wellness Center. Additionally, each participant will be provided with a Fitbit Zip to monitor daily activity and steps. Data from the Fitbit Zip will be downloaded weekly basis. Participants will be given a healthy step goal of 7000 steps per day. Based on initial step counts after the first week of wearing the accelerometer, individual goals will be discussed with the participants. Personal limitations that develop during the study period (i.e. peripheral neuropathy, orthopedic limitation, etc.) will be monitored and will be reason for exclusion from the exercise program. All sessions will be supervised and adherence to the exercise prescription will be fully monitored under the supervision and care of Dr. Stefany Primeaux in consultation with Dr. Neil Johannsen.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
68
Inclusion Criteria
  1. HIV positive
  2. Fasting glucose above 94 mg/dL and below 125 mg/dl
  3. Without diagnosis of diabetes mellitus
  4. "No" response to six questions in Physical Activity Readiness Questionnaire (PAR-Q), given at time of screening
Exclusion Criteria
  1. Lack of informed consent
  2. Decisionally impaired individuals
  3. Non-English speaking
  4. Acute illness within the preceding six weeks (defined as fever, new antibiotic use or unscheduled healthcare visit (for illness))
  5. Acute alcohol intoxication
  6. Pregnancy
  7. Currently prescribed blood thinners (if undergoing muscle biopsy)
  8. Allergy to Lidocaine (if undergoing muscle biopsy)
  9. Significant peripheral neuropathies (if undergoing exercise)
  10. Significant cardiovascular impairments (if undergoing exercise)

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
ExerciseExerciseAerobic exercise program using treadmill.
Primary Outcome Measures
NameTimeMethod
Fasting plasma glucose and OGTT values (subclinical dysglycemia)Baseline

Risk for subclinical dysglycemia

Secondary Outcome Measures
NameTimeMethod
myomiRsChange from baseline in myomiRs after 10 weeks

Dysfunctional SKM measured by myomiRs

Skeletal muscle phenotypeBaseline

Subclinical dysglycemia associated with dysfunctional skeletal muscle measured by myomiRs

Fasting plasma glucoseChange from baseline in fasting plasma glucose after 10 weeks

Glycemic control measured by fasting plasma glucose

Trial Locations

Locations (1)

Louisiana State University Health Sciences Center

🇺🇸

New Orleans, Louisiana, United States

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