Seminal Level of Clusterin in Infertile Men as a Predictor for Spermatogenesis Before Testicular Sperm Extraction
试验速览
- 阶段
- 不适用
- 入组人数
- 88
- 主要终点
- The level of seminal clusterin before testicular sperm Extraction.
研究概览
简要总结
Measurement of clusterin level in the semen of infertile males undergoing testicular sperm extraction.
详细描述
Clusterin, known as apolipoprotein J, sulphated glycoprotein-2 or testosterone - repressed prostate message-2.
It plays important roles in several pathophysiological processes, including tissue remodelling, lipid transport, reproduction, complement regulation and apoptotic cell death .
As clusterin expression is markedly upregulated in various normal and malignant tissues undergoing apoptosis, it has been regarded as a marker for cell death.
There is a conflicting findings concerning the relationship between elevated clusterin expression and enhanced induction of apoptosis; that is, clusterin has appeared to have a powerful anti-apoptotic function through a chaperone-like activity.
In addition to the anti-apoptotic activity, seminal clusterin was reported to promote a tolerogenic response to male antigens, thereby contributing to female tolerance to seminal antigens.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 50 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Infertile males , Azoospermia undergoing TESE as a preliminary step for ICSI .
- •Age: 20-50 year
排除标准
- •males <20 years and >50years
- •Cryptorchidism .
- •Testicular Agenesis and testicular atrophy .
结局指标
主要结局
The level of seminal clusterin before testicular sperm Extraction.
时间窗: 1 hour
Measurement of seminal clusterin by enzyme-linked immunosorbent assay (ELISA) and correlate them with the outcome of testicular sperm extraction to recognize predictors for spermatogenesis before testicular sperm extraction.
次要结局
未报告次要终点
研究者
Azza Sheshaey Hassan Abdelfadel Ahmad
Principal investigator
Assiut University