Skeletal Muscle Myokine Response to Acute Eccentric Exercise

Not Applicable

Recruiting

• Conditions: Skeletal Muscle

• Registration Number: NCT06677749
• Lead Sponsor: University of Manitoba

Brief Summary

The purpose of this study is to evaluate the acute local skeletal muscle inflammatory response to thigh muscle (quadriceps) eccentric muscle actions. Eccentric muscle actions occur when the muscle is lengthening as it contracts. This is a pilot project to assess the degree of inflammatory biomarkers, called myokines, that are released locally in skeletal muscle tissue after an acute bout of resistance-exercise using a resistance-exercise machine called an isokinetic dynamometer.

Detailed Description

The intervention will involve one session of maximal eccentric exercise of the knee extensor muscles (quadriceps) of the right leg. During the 24-hour period preceding the intervention, participants will be asked to refrain from any for of moderate-vigorous physical activity or exercise. Participants will perform 30 sets of 10 repetitions of maximal isokinetic eccentric knee extensions at 60 degrees/second with 1-minute of rest between each set. Before completing the exercise bout, each participant will be asked to remain in a seated position (resting) for 30-minutes and then provide a skeletal muscle biopsy using the modified Bergstrom with suction muscle biopsy technique from the vastus lateralis on the right leg. This will be done for the baseline assessment of 5 myokines including apelin, fibroblast growth factor-21, irisin, interleukin-6, and interleukin-15. Then the participants will complete the exercise bout as described above. They will then rest quietly in the laboratory for 3-hours and wait for the second skeletal muscle biopsy to be taken following this 3-hour wait.

Study Timeline

• Status Verified: 2024-07
• Study First Submitted: 2024-07-05
• Study First Submitted QC: 2024-11-04
• Last Update Submitted: 2024-11-04
• Study First Posted: 2024-11-07
• Last Update Posted: 2024-11-07
• Study Start: 2024-11-15
• Primary Completion: 2025-07-31
• Study Completion: 2025-11-01

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• 18-35 years old
• healthy
• recreationally active (>/= 150 minutes of moderate to vigorous physical activity per week for at least the past three months)

Exclusion Criteria

• "yes" answer to the Get Active Questionnaire which screens for health conditions that may affect exercise ability

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Primary Outcome Measures

Name	Time	Method
Apelin	Baseline and 3-hours post-exercise	Concentration of apelin in skeletal muscle tissue
Fibroblast growth factor-21	Baseline and 3-hours post-exercise	Concentration of fibroblast growth factor-21 in skeletal muscle tissue
Irisin	Baseline and 3-hours post-exercise	Concentration of irisin in skeletal muscle tissue
Interleukin-6	Baseline and 3-hours post-exercise	Concentration of interleukin-6 in skeletal muscle tissue
Interleukin-15	Baseline and 3-hours post-exercise	Concentration of interleukin-15 in skeletal muscle tissue

Secondary Outcome Measures

Name	Time	Method
Fat mass	Baseline	Fat mass in kilograms
Fat free mass	Baseline	Fat free mass in kilograms
Body mass	Baseline	Body mass in kilograms
Body height	Baseline	Body height in meters
Body mass index	Baseline	Body mass index in kg/m\^2

Trial Locations

Locations (1)

Applied Research Centre, Faculty of Kinesiology, University of Manitoba; 🇨🇦 Winnipeg, Manitoba, Canada