Measuring survival of transfused red blood cells in patients with Sickle cell disease and ß-Thalassemia: A Biotin Label approach
Recruiting
- Conditions
- Sickle cell disease & ß-thalassemia
- Registration Number
- NL-OMON25329
- Lead Sponsor
- Amsterdam UMC - location AMC
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 15
Inclusion Criteria
•Patient with either sickle cell disease (HbSS/HbSß0/HbSC/HbSß+) or ß-thalassemia (major or transfusion dependent thalassemia intermedia)
•Currently on chronic transfusion program.
•>18 years of age
Exclusion Criteria
No venous access for blood drawings
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method The primary outcome is the survival of transfused RBCs in patients with sickle cell disease and ß-thalassemia.
- Secondary Outcome Measures
Name Time Method Inter patient study parameters will focus on the effects of the transfusion. The main parameters will be:<br>•Inflammatory/immunomodulatory response on transfused RBCs.<br>•Influence of previous responder status on RBC survival.<br>•Antibody formation to the biotin label.<br><br>Intra patient study parameters will focus on the effect of matching of the RBC units. Both units will be matched differentially. <br>The main parameters will be:<br>•The recovery, short term and long term survival of both RBC units.<br>•The (immediate and late) host immune response to both RBC units by measuring effect on expression of markers of clearance and inflammation as well as transcriptome analysis.<br>