Effect of Aging & Obesity With Exercise Intervention

Not Applicable

Completed

• Conditions: Obesity
• Interventions: Other: Resistance exercise with a gym stick

• Registration Number: NCT03951857
• Lead Sponsor: Seoul National University Bundang Hospital

Brief Summary

Obesity leads to a vicious circle that increases intramuscular fat, insulin resistance, promotes muscular dysfunction resulting in increased muscle fat accumulation. The study of muscle function and intramyocellular lipids is insufficient for obesity. Particularly, the study of mechanisms in muscle function and intramyocellular lipids is few nationally and internationally.

Detailed Description

This study is prospective study. The goal of this study is

1. To examine the distribution and contractile properties (cross-sectional area, maximal force, specific force, maximal shortening velocity) of single muscle fiber sex/Myosin heavy chain (MHC) type-related differences, by extracting a single muscle fiber from the vastus lateralis in young men and women.

2. To examine the effects of intramyocellular lipids on physical activity ability, muscle dysfunction, and metabolic disorders in relation to aging and obesity.

3. To examine the effects of intramyocellular lipids on physical activity ability, muscular dysfunction, and metabolic disorders after 12 weeks resistance exercise intervention.

Study Timeline

• Study Start: 2016-11-18
• Study First Submitted: 2019-05-10
• Study First Submitted QC: 2019-05-14
• Study First Posted: 2019-05-15
• Primary Completion: 2020-02-29
• Study Completion: 2020-02-29
• Status Verified: 2020-03
• Last Update Submitted: 2020-03-10
• Last Update Posted: 2020-03-11

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 28

Inclusion Criteria

• Young men: Age (20-35 yrs), BMI (19-23 Kg/m2)
• Young women: Age (20-35 yrs), BMI (19-23 Kg/m2)
• Elderly women: Age (65-80 yrs), BMI (19-23 Kg/m2)
• Elderly obese women: Age (65-80 yrs), BMI ( >25 Kg/m2)
• Subjects who can agree voluntarily.

Exclusion Criteria

• Acute coronary syndrome.
• Uncontrolled hypertension.
• Subjects who took drugs which can affects neuromuscular system.
• Severely impaired cognition
• Subjects who cannot agree voluntarily

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Elderly women (control)	Resistance exercise with a gym stick	Range of age is 65-80 years, Elderly women (BMI 19-23 Kg/m2) They did not suffer from musculoskeletal or metabolic diseases, and had not performed regular exercise within the previous 3 months.
Elderly obese women	Resistance exercise with a gym stick	Range of age is 65-80 years,Elderly obese women (BMI ≥25 Kg/m2) They did not suffer from musculoskeletal or metabolic diseases, and had not performed regular exercise within the previous 3 months.

Primary Outcome Measures

Name	Time	Method
Single muscle fiber maximal force (Po)	Before intervention / after 12weeks intervention	Change from baseline Po to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber Po (mN) is measured by slack test procedure. after measurement, Po is analyzed to compare the change after intervention from baseline.
Total droplet area	Before intervention / after 12weeks intervention	Change from baseline total droplet area to 12 weeks after initial assessment. Total droplet area is determined by Oil-red-O staining. After staining, the total area (µm2) is analyzed to compare the change after intervention from baseline.
Number of lipid droplets	Before intervention / after 12weeks intervention	Change from baseline number of lipid droplets to 12 weeks after initial assessment.; Number of lipid droplets determined by Oil-red-O staining. After staining, number of lipid droplets is analyzed to compare the change after intervention from baseline.
Single muscle fiber specific force (SF)	Before intervention / after 12weeks intervention	Change from baseline SF to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber SF (kN/m2) is Po (kN) normalized by cross-sectional area (m2). SF is analyzed to compare the change after intervention from baseline.
Single muscle fiber MHC type distribution	Before intervention / after 12weeks intervention	Change from baseline fiber type distribution to 12 weeks after initial assessment.; The MHC composition of single fibers was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).; MHC type distribution is analyzed to compare the change after intervention from baseline.
Single muscle fiber cross-sectional area (CSA)	Before intervention / after 12weeks intervention	Change from baseline CSA to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber CSA (µm2) is measured by 3 dimensional microscopy. after analysis, CSA is compared the change after intervention from baseline.
Single muscle fiber maximal shortening velocity (Vo)	Before intervention / after 12weeks intervention	Change from baseline Vo to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber Vo (FL/s) is measured by slack test procedure. The time required to take up the imposed slack was measured from the onset of the length step to the beginning of the tension redevelopment . For each amplitude of length, the fiber was reextended while relaxed to minimize nonuniformity of sarcomere length. A straight line was presented to a plot of length vs. time, using least-squares regression, and the slope of the line divided by the segment length was recorded as Vo for that fiber.; Vo is analyzed to compare the change after intervention from baseline.

Secondary Outcome Measures

Name	Time	Method
Hand grip strength	Before intervention / after 12weeks intervention	Change from baseline hand grip strength to 12 weeks after initial assessment. Participants are tested while they were seated, their arms are against their sides, their elbows are flexed 90 degrees. Hand grip Strength is measured in kilograms (kg) using a hand-grip dynamometer. The maximum value from either hand is used for analysis.
Isokinetic knee extension strength	Before intervention / after 12weeks intervention	Change from baseline Knee extension strength to 12 weeks after initial assessment.; Isokinetic knee extension strength is measured in as peak torque achieved on an isokinetic dynamometer (BTE Primus, BTE tech, MD, USA) at 60° per second.

Trial Locations

Locations (1)

Seoul National University Bundang Hospital; 🇰🇷 SeongNam, Gyeonggi, Korea, Republic of