The Association between Chlamydia infection and Extrahepatic Biliary Atresia, a case control study
Completed
- Conditions
- bile duct atresiavanishing bile ducts10019806
- Registration Number
- NL-OMON38798
- Lead Sponsor
- Academisch Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 68
Inclusion Criteria
* Biopsy proven biliary atresia
* Underwent Kasai operation
* Liver tissue stored at the department of pathology
* Outpatient follow up in the Academic Medical Center, Amsterdam or University Medical Centre Groningen
* Younger then 12 years
* Informed consent of custodial parent(s) or guardian, for inclusion of their child
* Informed consent of the mother for the withdrawal of maternal blood for ELISA on
Chlamydia
Exclusion Criteria
* Patients with embryonic biliary atresia
* Older then 12 years
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The presence of Chlamydia IgA antibody in serum of Dutch patients with biliary<br /><br>atresia, compared to the control group.<br /><br>The presence of Chlamydia in liver tissue (liver biopsy and fibrotic remnant<br /><br>obtained during Kasai operation) of BA patients as compared to controls,<br /><br>examined by PCR as well as Chlamydia-specific staining by immunohistochemistry.<br /><br>In case indications for DNA abnormalities will arise, the remainder of the<br /><br>serum will be used for DNA analysis.</p><br>
- Secondary Outcome Measures
Name Time Method <p>The presence of Chlamydia IgA antibody in serum of mothers of patients with<br /><br>biliary atresia, compared to the presence of Chlamydia IgA antibody in serum of<br /><br>mothers of the control group and the presence of Chlamydia IgA antibody in the<br /><br>general population.</p><br>