Resistance Training and Hydrolyzed Collagen Supplementation in Healthy Young Adults

Not Applicable

Completed

• Conditions: Exercise Training; Nutrition; Healthy Participants
• Interventions: Dietary Supplement: Resistance training with hydrolyzed collagen ingestion in healthy young women; Dietary Supplement: Resistance training with hydrolyzed collagen ingestion in healthy young men

• Registration Number: NCT06372080
• Lead Sponsor: Liverpool John Moores University

Brief Summary

The goal of this clinical trial is to investigate the effects of resistance training with hydrolyzed collagen ingestion on changes in muscle and tendon adaptation in healthy young men and women.

The main questions it aims to answer are:

* Does resistance training with hydrolyzed collagen ingestion lead to greater changes in tendon properties than resistance training alone?

* Does resistance training with hydrolyzed collagen ingestion lead to greater changes in muscle size than resistance training alone?

Participants will be randomly assigned to collagen or placebo groups. Participants will perform resistance training three times per week for 10 weeks and hydrolyzed collagen or maltodextrin will be given to collagen or placebo group respectively immediately before each resistance training session. Also, vitamin C will be given to both groups.

Researchers will compare collagen and placebo groups to see if hydrolyzed collagen ingestion with resistance exercise would have beneficial effects on changes in muscle and tendon more than resistance training alone. Therefore, using isokinetic dynamometer and ultrasonography, maximal leg strength, morphological, mechanical, and material properties of the patellar tendon and vastus lateralis muscle size and architecture will be assessed.

Detailed Description

Young, healthy, active men and women will ingest 30 grams of hydrolyzed collagen or a calorie matched beverage (maltodextrin) alongside vitamin C prior to performing high-intensity resistance training 2 - 3 times per week for 10 weeks.

The aim of this study is to investigate the effect of combining hydrolyzed collagen supplementation with resistance training in young men and women. If supplementation with hydrolyzed collagen leads to a greater change in tendon size, stiffness, and Young's modulus than resistance training alone, this will allow us to recommend this type of intervention to young athletes seeking to improve tendon health and/or athletic performance.

The experimental design and measurements will be the same in the Arm 1 and Arm 2 but the differences will be sex where participants in Arm 1 will be healthy young men while participants in Arm 2 will be healthy young women.

Study Timeline

• Study Start: 2020-01-06
• Primary Completion: 2021-09-29
• Study Completion: 2023-03-31
• Status Verified: 2024-02
• Study First Submitted: 2024-04-03
• Study First Submitted QC: 2024-04-12
• Last Update Submitted: 2024-04-12
• Study First Posted: 2024-04-17
• Last Update Posted: 2024-04-17

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 23

Inclusion Criteria

• Healthy young male or female
• No history of patellar tendon injuries in the past 6 months
• No history of lower limb musculoskeletal injuries in the past 6 months
• Non-smokers (including e-cigarettes)
• Free from cardiovascular and metabolic diseases
• Nullipara (a woman who has never given birth)

Exclusion Criteria

• Age less than 18 years or higher than 40 years
• Being vegan or vegetarian (due to the mammalian source of collagen)
• Consumption of nutritional supplementation that purportedly affects muscle-tendon adaptation or recovery (i.e. protein powder, vitamin C, collagen)
• BMI over 30 kg/m2
• Previous anterior cruciate ligament injury where the patellar tendon was used as a graft

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Resistance training with hydrolyzed collagen ingestion in healthy young women	Resistance training with hydrolyzed collagen ingestion in healthy young women	The intervention procedure is the same as for Arm 1 except that the participants will be healthy young women (not men).
Resistance training with hydrolyzed collagen ingestion in healthy young men	Resistance training with hydrolyzed collagen ingestion in healthy young men	Healthy young men, pair-matched by age, body mass, height, and baseline strength, will be randomly allocated to the collagen (COL) or placebo (PLA) group. Pre- and post-training assessments are patellar tendon properties, maximal knee flexor and extensor strengths and vastus lateralis muscle morphology using an isokinetic dynamometer and ultrasound scan. Participants will perform the 10-week progressive resistance training program three times per week and training loads will be adjusted weekly based on the prior session's performance. In each session, two different calorie-matched beverages were given immediately before the start of resistance training. 30 grams of hydrolyzed collagen (HC) and 30.5 grams of maltodextrin were used for COL and PLA respectively. HC or maltodextrin was mixed with 250 milliliters of water, 50 milligrams of vitamin C and 3 grams non-caloric sweetener which was used to mask flavour in an opaque bottle.

Primary Outcome Measures

Name	Time	Method
Patellar tendon cross-sectional area	12 weeks (10 weeks' intervention plus one week either side for pre- and post-intervention assessments)	Using ultrasonography, patellar tendon cross-sectional area (in millimeters squared) will be measured.
Patellar tendon stiffness	12 weeks (10 weeks' intervention plus one week either side for pre- and post-intervention assessments)	Using ultrasonography, patellar tendon stiffness (in Newtons per millimeter) will be measured.
Maximal knee extensor (quadriceps) muscle strength	12 weeks (10 weeks' intervention plus one week either side for assessments)	Using isokinetic dynamometer, knee extensor maximal isometric voluntary contraction torque (in Newton meters) will be measured.
Maximal knee flexor (hamstring) muscle strength	12 weeks (10 weeks' intervention plus one week either side for pre- and post-intervention assessments)	Using an isokinetic dynamometer, knee flexor maximal isometric voluntary contraction torque (in Newton meters) will be measured.
Vastus lateralis (VL) muscle fascicle pennation angle	12 weeks (10 weeks' intervention plus one week either side for pre- and post-intervention assessments)	Using ultrasonography, VL muscle fascicle pennation angle (in degrees) will be measured.
Vastus lateralis (VL) muscle size	12 weeks (10 weeks' intervention plus one week either side for pre- and post-intervention assessments)	Using ultrasonography, the VL anatomical cross-sectional area (in centimeters squared) will be measured.
Vastus lateralis (VL) muscle fascicle length	12 weeks (10 weeks' intervention plus one week either side for pre- and post-intervention assessments)	Using ultrasonography, VL muscle fascicle length (in millimeters) will be measured.

Trial Locations

Locations (1)

Research Institute for Sport and Exercise Sciences; 🇬🇧 Liverpool, Merseyside, United Kingdom