Relationship Between the VEGF Gene Polymorphism and Cardiometabolic Risk Factors
- Conditions
- Polymorphism, Restriction Fragment Length
- Interventions
- Genetic: gene expression
- Registration Number
- NCT04077554
- Lead Sponsor
- Poznan University of Medical Sciences
- Brief Summary
The aim of the study is to determine the relationship between VEGF gene expression, VEGF gene polymorphism and serum leptin concentration in the Polish population in people with excessive body weight. In addition, the aim of the study is to look for relationships between the VEGF gene polymorphism and anthropometric and biochemical factors of cardiovascular risk and endothelial dysfunction such as body weight, waist circumference, serum total cholesterol, LDL, HDL, triglycerides, glucose and the occurrence of cardiovascular diseases in the family of a patient with excessive body weight in the Polish population.
- Detailed Description
400 people (250 - study group; 150 control group) were subjected to subjective and objective action. Information on the occurrence of diseases and cardiovascular risk in the participant and his family was collected from research studies. Anthropometric parameters were measured (body weight, height, BMI, waist circumference, neck circumference) as well as blood pressure and pulse measurement. In addition, fasting venous blood was collected and secured. In venous blood currently marked with the following concentration: glucose, required cholesterol, LDL cholesterol, HDL cholesterol and triglycerides. In addition, a method of salting out and protecting DNA was developed from blood.
Furthermore the polymorphisms of VEGF genes (in positions: -2578 and -634) using the HMR (High Resolution Melt) method was determined.
Also VEGF and leptin by ELISA was determined. After obtaining the results of comparative analysis of the correlation between the occurrence of VEGF gene polymorphisms, serum levels of leptin and VEGF, and anthropometric and biochemical parameters of cardiovascular risk.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 400
- informed consent in writing
- stable body mass (+/- 1 kg)
- excessive body mass (BMI ≥ 25 kg/m2- study group)
- proper body mass (BMI < 25 kg/m2- control group)
- age <18 years
- secondary obesity
- pregnancy, lactation
- other conditions that in the opinion of researchers may pose any risk to the patient during the study.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Control group gene expression women and men with proper body mass Study group gene expression women and men with excess body mass
- Primary Outcome Measures
Name Time Method VEGF gene expression through study completion, an average of 1 year VEGF gene expression
VEGF gene polymorphism through study completion, an average of 1 year VEGF gene polymorphism
serum leptin concentration through study completion, an average of 1 year serum leptin concentration
- Secondary Outcome Measures
Name Time Method Body mass (BM) through study completion, an average of 1 year Body mass
Waist circumference (WC) through study completion, an average of 1 year Waist circumference
low density lipoprotein (LDL) through study completion, an average of 1 year Blood concentration of low density lipoprotein
total cholesterol (TCH) through study completion, an average of 1 year Blood concentration of total cholesterol
high density lipoprotein (HDL) through study completion, an average of 1 year Blood concentration of high density lipoprotein
triglycerides through study completion, an average of 1 year Blood concentration of triglycerides
glucose through study completion, an average of 1 year Blood concentration of glucose
Trial Locations
- Locations (1)
Department of Treatment of Obesity, Metabolic Disorders and Clinical Dietetics, Poznan University of Medical Sciences
🇵🇱Poznan, Wielkopolska, Poland