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Butyrate-enriched Triglyceride and Diabetes Prevention

Not Applicable
Recruiting
Conditions
PreDiabetes
Type 2 Diabetes
Obesity
Interventions
Dietary Supplement: butyrate/hexanoate-enriched triglycerides
Registration Number
NCT06384313
Lead Sponsor
Maastricht University Medical Center
Brief Summary

A body of animal studies as well as observational studies in humans demonstrated that butyrate is one SCFA that has pronounced positive effects on body weight control, inflammation, and insulin resistance. Even though the SCFA hexanoate is less researched, it has been shown to be involved in anti-inflammatory processes. Of note, acute human studies showed that fibre-induced metabolic improvements are linked to higher SCFA levels in the systemic circulation. It has been shown that a butyrate/hexanoate-enriched triglyceride oil enhanced systemic butyrate and hexanoate concentrations for a prolonged time. Yet, it remains to be determined whether a chronic increase in circulating butyrate and hexanoate concentrations translate into long-term benefits. In this study it is hypothesized that a chronic increase of butyrate/hexanoate in the circulation may improve host metabolism and metabolic health by improving adipose tissue function, reducing systemic lipid overflow and inflammation thereby increasing peripheral insulin sensitivity in individual with overweight/obesity and prediabetes.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
48
Inclusion Criteria

In order to be eligible to participate in this study, a subject must meet all of the following criteria:

  • Age 20-70 years

  • BMI ≥ 28 and < 40 kg/m2

  • Weight stable for at least 3 months

  • Normal blood pressure (systolic blood pressure 100-140 mmHg, diastolic blood pressure 60-90 mmHg)

  • One or more of the following criteria to determine disturbed glucose/insulin homeostasis

    • Fasting glucose 5.6-6.9 mmol/L
    • Two-hour glucose of 7.8-11.1 mmol/L
    • HOMA-IR ≥ 2.2
    • HbA1c (5.7-6.4%)
Exclusion Criteria
  • Diabetes mellitus (type 1 or 2)

    • Cardiovascular disease: including no history or myocardial infarction, heart failure, arrhythmias
    • Pulmonary disease: no history of chronic obstructive pulmonary disease, emphysema, bronchitis, asthma
    • Kidney (e.g. kidney failure) or liver (e.g. cirrhosis, non-alcoholic fatty acid) malfunction
    • Gastrointestinal disease (no inflammatory bowel disease, irritable bowel syndrome or digestive disorders) or a history of abdominal surgery (except appendectomy and cholecystectomy)
    • Autoimmune disease
    • Any other diseases affecting glucose and/or lipid metabolism or use of any medication that influence glucose or fat metabolism and inflammation
    • Ongoing disease or any disease with a life expectancy ≤ 5 years
    • Abuse of products; alcohol (>15 units per week) and drugs, excessive nicotine use defined as >20 cigarettes per week
    • Regular supplementation of pre- or probiotic products, use of pre- or probiotics, antibiotics and laxatives 3 months prior to the start of the study
    • Intensive exercise training more than three hours a week
    • Plan to lose weight or to follow a hypocaloric diet or vegetarian diet
    • Pregnancy

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
butyrate/hexanoate-enriched triglyceridesbutyrate/hexanoate-enriched triglyceridesOil containing butyrate and hexanoate-enriched triglycerides
placebobutyrate/hexanoate-enriched triglyceridesOil devoid of butyrate and hexanoate but with similar composition as the intervention oil
Primary Outcome Measures
NameTimeMethod
Peripheral insulin sensitivityBefore and 6 months after the start of the intervention

The change of peripheral insulin sensitivity as assessed by a hyperinsulinaemic-euglycemic clamp

Secondary Outcome Measures
NameTimeMethod
circulating SCFABefore and 6 months after the start of the intervention

The change in concentrations of circulating SCFA

body weightBefore and 6 months after the start of the intervention

The change in body weight in kg using a weight scale

hepatic insulin sensitivityBefore and 6 months after the start of the intervention

The change in hepatic insulin sensitivity as assessed by a hyperinsulinaemic-euglycemic clamp

Substrate oxidation (indirect calorimetry)Before and 6 months after the start of the intervention

The change in substrate oxidation as measured via ventilated hood system

Faecal SCFABefore and 6 months after the start of the intervention

The change in concentrations of faecal SCFA

Faecal microbiota compositionBefore and 6 months after the start of the intervention

The change in faecal microbiota composition assessing abundances of bacteria and diversity indices as assessed via 16s rRNA gene

adipose tissue insulin sensitivityBefore and 6 months after the start of the intervention

The change in adipose tissue insulin sensitivity as assessed by a hyperinsulinaemic-euglycemic clamp

Energy expenditure (indirect calorimetry)Before and 6 months after the start of the intervention

The change in energy expenditure as measured via ventilated hood system

body compositionBefore and 6 months after the start of the intervention

The change in body composition as assessed using DEXA scans

Circulating hormones such as insulinBefore and 6 months after the start of the intervention

The change in concentrations of circulating hormones in peripheral blood

Circulating metabolites such as glucoseBefore and 6 months after the start of the intervention

The change in concentrations of metabolites in peripheral blood

Gut permeabilityBefore and 6 months after the start of the intervention

The change in gut permeability as assessed using a multi-sugar test

Trial Locations

Locations (1)

Maastricht University

🇳🇱

Maastricht, Limburg, Netherlands

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