Skeletal muscle mitochondrial (dys)function in humans: Relating macroscopic in vivo observations to mechanistic understanding at molecular scale

Completed

Conditions: veroudering, mitochondriële myopathie
aging
diabetes
10012653

Registration Number: NL-OMON39110

Lead Sponsor: Maxima Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 52

Inclusion Criteria

All subjects:
- written informed consent;Mitochondrial myopathy:
- patients diagnosed with mitochondrial myopathy
- Age: 18+;Type 2 diabetes:
- patients diagnosed with Type 2 diabetes for at least 2 years
- age: 18+
- BMI 26 - 42 kg/m2
- sedentary behaviour as confirmed by a questionnaire asking about physical activity levels
- sex: male

Elderly with sedentary lifestyle:
- Age: 50 - 75 yrs
- sedentary lifestyle as confirmed by a questionnaire asking about physical activity levels
- sex: male ;Elderly with active lifestyle:
- Age: 50 - 75 yrs
- active lifestyle as confirmed by a questionnaire asking about physical activity levels
- sex: male

Control subjects:
- Age: 20 - 30 yrs
- BMI: 20 - 25
- Normally active lifestyle as confirmed by a questionnaire asking about physical activity levels
- sex: male

Exclusion Criteria

All subjects:
- pacemaker / implantable cardioverter defibrillator (ICD)
- metal prostheses
- implanted devices
- vascular clips
- profession (current of in the past) as welder or metalworker (risk of small metal fragments in eyes);- claustrophobia
- use of oral anti-coagulants
- use of inorganic nitrate
- cardio-vascular disease (decompensatio cordis, agina pectoris, myocardial infarction of positive signs of cardiac ischaemia on the ECG during the incremental exercise test on the bicycle ergometer)
- cerebro-vascular disease (CVA)
- neurological diseases of deficits
- vascular complications

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Mitochondrial function will be assessed by 31P magnetic resonance spectroscopy<br /><br>(rate of PCr recovery after submaximal exercise) in M. vastus lateralis.<br /><br>Differences in molecular phenotype of the muscle cells will be obtained by<br /><br>analysis of muscle biopsy samples for:<br /><br>- State 3, 4, U respiration<br /><br>- Complex I, II, III, IV, ATPase, ANT, UCP3 content<br /><br>- Mitochondrial DNA copy number<br /><br>- Fiber type composition, muscle vascularisation </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- VO2max en Qmax determined on calibrated bicycle ergometer<br /><br>- Relation between oxygen consumption and power output determined on calibrated<br /><br>bicycle ergometer</p><br>