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Long-term Cognitive and Cerebral Changes in Sepsis Survivors and Their Predictors

Not Applicable
Conditions
Severe Sepsis With Septic Shock
Severe Sepsis Without Septic Shock
Interventions
Procedure: Blood Sample
Behavioral: Neurocognitive Assessment
Other: Resting State EEG
Procedure: Lumbar Puncture
Other: MRI
Registration Number
NCT02339649
Lead Sponsor
German Center for Neurodegenerative Diseases (DZNE)
Brief Summary

The main goals of this study are to provide a cognitive, neurological, brain morphological, and serological profile of sepsis survivors in order to make long-term prognosis of recovery and estimate the need for rehabilitation measures in order to help patients reintegrate into normal daily life.

Detailed Description

Comparison of cognitive function in severe septic/septic shock ICU patients with non-septic postoperative ICU patients and actively recruited healthy controls at several time points up to 12 months after leaving the ICU.

Secondary aims:

* Profiling specific cognitive deficits in sepsis survivors over time.

* Identifying possible subclasses of long-term cognitive impairment according to facets of disease, therapy and ICU experience.

* Investigating long-term brain morphological changes, with hippocampus as region of interest (ROI).

* Determining values of Serum- and CSF-Biomarkers

* Evaluation of diagnostic and prognostic value of non-routine biomarkers for systemic- and neuroinflammation in the acute phase and in the long-term

* Investigate associations between brain morphological changes, cognitive changes, neurological status, psychiatric burden, disability level, employment status and health-related quality of life (HRQOL).

* Investigating brain-activity changes in resting state electro-encephalogram (EEG)

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
308
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Postoperative ICU PatientsResting State EEGMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80: * Admitted to the intensive care units of the Anesthesiological-Operative Intensive Care Unit of the University Hospital Bonn, which includes a surgical ICU, an anesthesiological ICU, and a cardio-surgical ICU * Duration of ICU stay must be a minimum of 24 hours. * Mini-Mental State Examination (MMSE) Score of 25 or above Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Healthy ControlsNeurocognitive AssessmentHealthy Controls will only be included in the study if they meet all of the following criteria: Written informed consent of the subject, Aged 25-80 years, Male or female. Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Healthy ControlsResting State EEGHealthy Controls will only be included in the study if they meet all of the following criteria: Written informed consent of the subject, Aged 25-80 years, Male or female. Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Sepsis/septic ShockBlood SampleMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80; fulfill the criteria of sepsis and/or septic shock patients according to theThird International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3, Singer et al, 2016). Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Postoperative ICU PatientsMRIMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80: * Admitted to the intensive care units of the Anesthesiological-Operative Intensive Care Unit of the University Hospital Bonn, which includes a surgical ICU, an anesthesiological ICU, and a cardio-surgical ICU * Duration of ICU stay must be a minimum of 24 hours. * Mini-Mental State Examination (MMSE) Score of 25 or above Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Healthy ControlsMRIHealthy Controls will only be included in the study if they meet all of the following criteria: Written informed consent of the subject, Aged 25-80 years, Male or female. Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Sepsis/septic ShockResting State EEGMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80; fulfill the criteria of sepsis and/or septic shock patients according to theThird International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3, Singer et al, 2016). Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Sepsis/septic ShockMRIMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80; fulfill the criteria of sepsis and/or septic shock patients according to theThird International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3, Singer et al, 2016). Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Postoperative ICU PatientsLumbar PunctureMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80: * Admitted to the intensive care units of the Anesthesiological-Operative Intensive Care Unit of the University Hospital Bonn, which includes a surgical ICU, an anesthesiological ICU, and a cardio-surgical ICU * Duration of ICU stay must be a minimum of 24 hours. * Mini-Mental State Examination (MMSE) Score of 25 or above Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Healthy ControlsBlood SampleHealthy Controls will only be included in the study if they meet all of the following criteria: Written informed consent of the subject, Aged 25-80 years, Male or female. Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Sepsis/septic ShockNeurocognitive AssessmentMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80; fulfill the criteria of sepsis and/or septic shock patients according to theThird International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3, Singer et al, 2016). Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Sepsis/septic ShockLumbar PunctureMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80; fulfill the criteria of sepsis and/or septic shock patients according to theThird International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3, Singer et al, 2016). Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Postoperative ICU PatientsBlood SampleMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80: * Admitted to the intensive care units of the Anesthesiological-Operative Intensive Care Unit of the University Hospital Bonn, which includes a surgical ICU, an anesthesiological ICU, and a cardio-surgical ICU * Duration of ICU stay must be a minimum of 24 hours. * Mini-Mental State Examination (MMSE) Score of 25 or above Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Postoperative ICU PatientsNeurocognitive AssessmentMen and women seen at the intensive care units of the Anesthesiology Department of the University Hospital Bonn aged 25-80: * Admitted to the intensive care units of the Anesthesiological-Operative Intensive Care Unit of the University Hospital Bonn, which includes a surgical ICU, an anesthesiological ICU, and a cardio-surgical ICU * Duration of ICU stay must be a minimum of 24 hours. * Mini-Mental State Examination (MMSE) Score of 25 or above Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Healthy ControlsLumbar PunctureHealthy Controls will only be included in the study if they meet all of the following criteria: Written informed consent of the subject, Aged 25-80 years, Male or female. Interventions: Neurocognitive Assessment, Clinical Scales, Questionnaires, Blood Sample, voluntary Lumbar Puncture, Resting State EEG, MRI
Primary Outcome Measures
NameTimeMethod
Verbal Learning Memory Test Long Delayed Recall (Trial 7)6 months post ICU

Verbal Learning Memory Test Long Delayed Recall (Trial 7) at 6 months post ICU admission in sepsis/septic shock patients versus non-septic postoperative patients, and comparison to actively recruited healthy controls)

Secondary Outcome Measures
NameTimeMethod
German Vocabulary TestFor patient groups: 12 months; for Healthy Controls: 6 months

Premorbid Verbal Ability: German Vocabulary Test

Wechsler Memory Scale IV Visual Recall I and IIFor patient groups: 12 months; for Healthy Controls: 6 months

Visual Memory: Wechsler Memory Scale IV Visual Recall I and II

Verbal Learning Memory Test, German VersionFor patient groups: 12 months; for Healthy Controls: 6 months

Verbal Learning and Memory: Verbal Learning Memory Test, German Version

Wechsler Memory Scale Digit-SpanFor patient groups: 12 months; for Healthy Controls: 6 months

Working Memory: Wechsler Memory Scale Digit-Span

Symbol Digit Modalities TestFor patient groups: 12 months; for Healthy Controls: 6 months

Cognitive Speed

Trail Making Test A & BFor patient groups: 12 months; for Healthy Controls: 6 months

Simple and Divided Attention

Patient Health Questionnaire Subtests Depression, Anxiety and Somatic SymptomsFor patient groups: 12 months; for Healthy Controls: 6 months

Depression and Anxiety Inventory

Post-Traumatic Stress Syndrome 10For patient groups: 12 months; for Healthy Controls: 6 months

Post-Traumatic Stress Screening

MRI (3 Tesla) of brain with hippocampus as Region of InterestFor patient groups: 6 months, 12 months; for Healthy Controls: Baseline and 6 months

MRI (3 Tesla) of brain with hippocampus as Region of Interest. Protocol includes: three-dimensional, T1-weighted rapid gradient echo sequence, Resting State, Gradient Echo-Field-Map for Resting State, Inversion Recovery Echo Planar Imaging for Resting State, Spoiled Gradient Echo, Fluid Attenuated inversion Recovery, Susceptibility weighted imaging, Diffusion Tensor Imaging, Gradient Echo-Field-Map for Diffusion Tensor Imaging

Lumbar Puncture (voluntary)For Patients: (while at ICU, if clinically indicated as part of clinical routine and only if patient directly gives informed consent), 12 months; For Healthy Controls: Baseline and 6 months

Cerebrospinal fluid (CSF) routine parameters will be analyzed by the Central Laboratory. Results will then be provided to the investigator. For determination of τ, phospho-τ, β-amyloid 1 40 and β-amyloid 1 42, 5 ml of cerebral-spinal-fluid will be analyzed at the laboratories of the German Center for Neurodegenerative Diseases (DZNE), Biomarker Dementia Working Group, Sigmund-Freud-Str. 25, 53105 Bonn

Functional Activities QuestionnaireFor patient groups: 12 months; for Healthy Controls: 6 months

Activities of Daily Living

Clinical Scales at ICUFor Patients during ICU Stay (Day 0, [Day 7 if still on ICU], Day of Release)

Therapeutic Intervention Scoring System-10, Total Score Simplified Acute Physiology Score, Sequential Organ Failure Assessment Confusion Assessment Method for the ICU

Phonetic and Lexical Verbal FluencyFor patient groups: 12 months; for Healthy Controls: 6 months

Phonetic and Lexical Verbal Fluency

European Quality of Life 5 Dimensions-5 LevelsFor patient groups: 12 months; for Healthy Controls: 6 months

Quality of Life

modified version of Rankin Scale Structured Interview (modified for ICU patients)For patient groups: 12 months; for Healthy Controls: 6 months

Function Scale

Pittsburgh Sleep Quality IndexFor patient groups: 12 months; for Healthy Controls: 6 months

Sleep Quality

Neurological ExaminationFor patient groups: 6 months, 12 months; for Healthy Controls: Baseline and 6 months

Subscales Motor ability, Walking and Standing, Behavior, Praxis/Visual or Visuospatial, Speaking and Language, Autonomic Nervous System, Tone and Muscle Exam, Coordination and Movement

resting state electroencephalogramFor patient groups: 6 months, 12 months; for Healthy Controls: Baseline and 6 months

10-20 system electroencephalogram (64 channels with additional electro-oculogram, electromyogram, electrocardiogram). The protocol includes 5 phases, each one minute long: 1) eyes open, relaxed state, fixation of a black dot at 2 m distance, 2) word generation using letter "P" with eyes closed, 3) mental arithmetic: continuous subtraction of 7 starting from 1,000 with eyes closed, 4) eyes closed, relaxed state, no task, 5) visualization of a previously memorized geometric figure with eyes closed. Data sampled at a rate of 256 Herz using an anti-aliasing low pass filter. Power spectra will be calculated for consecutive 4-second windows and calculated for each electrode contact. Absolute spectral power will be determined for the delta (0.5-4 Herz), theta (4-8 Herz), alpha (8-13 Herz), beta (13-20 Herz) and gamma (20-40 Herz) bands, which will be averaged across all windows.

Mini Mental Status ExaminationFor patient groups: 12 months; for Healthy Controls: 6 months

Cognitive Screening

Blood SampleFor Patient Groups 2-3 samples during ICU, 3-4 Months, 6 months, 12 Months

All blood samples shall be drawn, stored and analyzed according to the participant information in the laboratories of the Biomarker Dementia Working Group and the Central Laboratory. Blood sampling analysis will include leukocyte count, hemoglobin, hematocrit, erythrocytes, Mean Corpuscular Hemoglobin, Meam Corpuscular Volume, mean Corpuscular Hemoglobin Concentration, Thrombocytes, Glucose, Lactate, electrolytes Sodium, Potassium, Calcium, Cholesterine, Tumor necrosis Factor-alpha, Interleukin-1 alpha, Interleukin-1 beta, Interleukin-6, C-reactive protein, Neuron Specific Enolase, Procalcitonin, S-100 Protein, as well as DNA and serum biorepository for further scientific analysis.

Trial Locations

Locations (2)

Department of Anesthesiology, University Hospital Bonn

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Bonn, North Rhine-Westphalia (NRW), Germany

DZNE Center for Clinical Research

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Bonn, Northrhine-Westphalia, Germany

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