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Immunoregulatory Therapy for 2019-nCoV

Phase 2
Conditions
2019 nCoV, PD-1
Interventions
Drug: Thymosin+standard treatment
Drug: PD-1 blocking antibody+standard treatment
Other: standard treatment
Registration Number
NCT04268537
Lead Sponsor
Southeast University, China
Brief Summary

Sepsis, including viral infections, are major causes of death worldwide. Studies show that in 2017, the number of sepsis patients worldwide reached as high as 48.9 million, of which 11 million patients died. Studies in China also showed that more than 1 million patients died of sepsis in 2015. Previous studies have suggested that sepsis are often secondary to excessive inflammatory response syndrome. However, treatment measures targeting excessive inflammatory response failed to effectively improve the prognosis of patients. PD-1 and PD-L1 are key mediators in T cell depletion in sepsis patients. Therefore, the investigators try to performe a clinical research to investigate the efficacy of PD-1 and thymosin in patients with severe pneumonia associated with lymphocytopenia in 2019 novel coronavirus infection.

Detailed Description

Sepsis, including viral infections, are major causes of death worldwide. Studies show that in 2017, the number of sepsis patients worldwide reached as high as 48.9 million, of which eleven million patients died. Studies in China also showed that more than one million patients died of sepsis in 2015. Therefore, how to effectively reduce the mortality of patients with sepsis has become a focus of clinical and basic research.

Previous studies have suggested that sepsis are often secondary to excessive inflammatory response syndrome. However, treatment measures targeting excessive inflammatory response failed to effectively improve the prognosis of patients. The reason is that sepsis-related immune dysfunction can increase the risk of secondary infection and even affect the fatality rate.

The immune checkpoint pathway is the endogenous component of the immune system, which is responsible for checking the immune response and keeping it in a normal physiological state. Tumor cells can evade host recognition through this pathway. One of these immunocheckpoint pathways is the PD-1 and PD-L1 pathways. PD-1 is a receptor expressed on the surface of T cells and ACTS as a negative regulator of T cell function. Monoclonal antibody blocking the activity of PD-1 can successfully reduce tumor load and has been widely used in the clinical treatment of various tumors.

The immune imbalance in patients with sepsis has many similarities tumors. PD-1 and PD-L1 are key mediators in T cell depletion in sepsis patients. Animal models have shown that blocking PD-1 or PD-L1 can prevent T cell death, regulate cytokine production, reduce organ dysfunction and reduce death in sepsis. Previous study showed the clinical safety of anti-PD-1 antibody in sepsis patients through randomized, placebo-controlled trials.

Thymosin has also been proved to regulate cellular immunity in sepsis patients. Some studies have shown that thymosin can significantly reduce the mortality of sepsis patients. At present, phase III clinical research is in progress to further clarify the role of thymosin in patients with sepsis. The purpose of this study was to investigate the efficacy of PD-1 and thymosin in patients with severe pneumonia associated with lymphocytopenia in 2019 novel coronavirus infection.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
120
Inclusion Criteria
  1. Adult SARI patients with 2019-ncov infection confirmed by PCR;
  2. Absolute value of lymphocytes < 0. 6x 109/L;
  3. Severe respiratory failure within 48 hours and requires admission to ICU. (severe respiratory failure was defined as PaO2/FiO2 < 200 mmHg and was supported by positive pressure mechanical ventilation (including non-invasive and invasive mechanical ventilation, PEEP>=5cmH2O))
Exclusion Criteria
  1. Age < 18
  2. Pregnant
  3. Allergic to experimental drugs
  4. The underlying disease is very serious and the expected survival time is less than 6 months (such as advanced malignant tumor);
  5. COPD or end-stage lung disease requires home oxygen therapy
  6. Expected survival time not exceeding 48 hours
  7. Participated in other clinical intervention trials within the last 3 months
  8. Autoimmune diseases
  9. A history of organ, bone marrow or hematopoietic stem cell transplantation 10. Received radiotherapy and chemotherapy for malignant tumor within 6 months

11.HIV infected patients or diagnosed with acquired immunodeficiency within the past year (CD4 T cells <=200/mm3) 12. Patients receiving anti-hcv treatment 13.90 days of retinal detachment or eye surgery 14. Permanent blindness in one eye 15. History of iritis, endophthalmitis, scleral inflammation or retinitis 16. The competent physician considered it inappropriate to participate in the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
thymosin groupThymosin+standard treatmentThymosin, 1.6 mg sc qd, last for 5 days
PD-1 groupPD-1 blocking antibody+standard treatmentAnti-PD-1 antibody, 200mg, IV, one time
control groupstandard treatmentstand treatment
Primary Outcome Measures
NameTimeMethod
lung injury score7 days

proportion of lung injury score decreased 1 or more points

Secondary Outcome Measures
NameTimeMethod
absolute lymphocyte counts7, 14 and 28 days

lymphocyte counts at day 7, 14 and 28 after randimization

serum level of CRP, PCT and IL-63, 7 and 14 days

serum level of CRP, PCT and IL-6 at day 3,7 and 14 after randimization

SOFA score7 days

SOFA score at Day 7, with scores range from 0 to 24 and higher score means worse outcome

all cause mortality rate28 days

died at day 28

ventilation free days28 days
ICU free daysup to 28 days
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