DNA Repair in Patients With Stable Angina.
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 172
- 试验地点
- 1
- 主要终点
- Association between monocytes exhibiting reduced base excision repair and/or double strand break repair activity in patients with stable angina as compared to patients without coronary artery disease.
研究概览
简要总结
Markers of DNA damage and repair are present in both atherosclerotic plaques and peripheral blood mononuclear cells of patients with coronary artery disease. A positive correlation has been observed between the level of DNA damage and the severity of atherosclerotic lesions, as well as atherogenic risk factors such as smoking, hypertension and hyperlipidaemia. A number of in-vitro studies have implicated defective DNA repair in the development and progression of atherosclerotic lesions. In mouse models of atherosclerosis, the DNA repair signalling cascade has been shown to be amenable to pharmacological intervention and overexpression of specific repair proteins attenuate the development of atherosclerotic plaques. However, data regarding the role of DNA repair in the pathogenesis of atherosclerosis in humans are lacking. We have preliminary data indicating reduced DNA repair activity in patients with stable angina. This study will determine the molecular basis and the biological consequences of this observation.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age ≥18 years
- •Ability to provide written informed consent
排除标准
- •Age ≥ 80 years
- •Inability to provide written informed consent
- •Presentation with an acute coronary syndrome
- •Severe valvular heart disease
- •Hypertrophic cardiomyopathy
- •Left ventricular ejection fraction ≤ 35%
- •Prior coronary revascularisation (surgical or percutaneous)
- •Diabetes Mellitus
- •Clinical evidence of peripheral vascular disease
- •Prior history of cerebrovascular disease
- •Malignancy
- •Active inflammatory disorders (e.g. rheumatoid arthritis/connective tissue disorder)
- •Renal impairment eGFR <60ml/min/1.73m2
- •Anaemia (Hb <100g/dL)
结局指标
主要结局
Association between monocytes exhibiting reduced base excision repair and/or double strand break repair activity in patients with stable angina as compared to patients without coronary artery disease.
时间窗: Blood will be collected at the time of hospital admission for index procedure. No follow-up of patients will be required.
This will be assessed using real-time polymerase chain reaction, western blotting and proteosomal degradation assay.
Reduced DNA repair activity is associated with impaired response to oxidative stress in human monocytes in patients with stable angina in comparison to an age and gender matched control.
时间窗: Blood will be collected at the time of hospital admission for index procedure. No follow-up of patients will be required.
次要结局
未报告次要终点