The effect of a high-fat meal on the immune system
- Conditions
- atherosclerosisinnate immune memoryhigh-fat challengeslagaderverkalkinggeheugen van het aangeboren afweersysteemvetrijke maaltijd
- Registration Number
- NL-OMON24430
- Lead Sponsor
- Radboud University Medical Center
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 15
Inclusion Criteria
Age between 18 and 40 years
- LDL cholesterol < 3.5 mmol/l, fasting triglycerides < 2 mmol/l
Exclusion Criteria
- Smoking within the year before study entry
- Diagnosed with any long-term medical condition that can interfere with the study (i.e. renal failure, cardiovascular disease, diabetes, rheumatoid arthritis etc.)
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method The primary endpoint is the TNFa production upon ex vivo stimulation with LPS of monocytes isolated 72h after the consumption of high-fat shake.
- Secondary Outcome Measures
Name Time Method Additional secondary endpoints are the production of other cytokines and chemokines upon ex vivo stimulation, the monocytes’ inflammatory phenotype as assessed by flowcytometry analysis , epigenetic and metabolic reprogramming and serum induced inflammation.